Journal article
Localization of complement 1 inhibitor (C1INH/SERPING1) in human eyes with age-related macular degeneration
Experimental Eye Research, Vol.89(5), pp.767-773
11/2009
DOI: 10.1016/j.exer.2009.07.001
PMCID: PMC2757497
PMID: 19607829
Abstract
Age-related macular degeneration (AMD) is a common degenerative disease resulting in injury to the retina, retinal pigment epithelium and choriocapillaris. Recent data from histopathology, animal models and genetic studies have implicated altered regulation of the complement system as a major factor in the incidence and progression of this disease. A variant in the gene SERPING1, which encodes C1INH, an inhibitor of the classical and lectin pathways of complement activation, was recently shown to be associated with AMD. In this study we sought to determine the localization of C1INH in human donor eyes. Immunofluorescence studies using a monoclonal antibody directed against C1INH revealed localization to photoreceptor cells, inner nuclear layer neurons, choriocapillaris, and choroidal extracellular matrix. Drusen did not exhibit labeling. Genotype at rs2511989 did not appear to affect C1INH abundance or localization, nor was it associated with significant molecular weight differences when evaluated by Western blot. In a small number of eyes (n = 7 AMD and n = 7 control) AMD affection status was correlated with increased abundance of choroidal C1INH. These results indicate that C1INH protein is present in the retina and choroid, where it may regulate complement activation.
Details
- Title: Subtitle
- Localization of complement 1 inhibitor (C1INH/SERPING1) in human eyes with age-related macular degeneration
- Creators
- Robert F Mullins - Carver Family Center for Macular Degeneration, Department of Ophthalmology and Visual Sciences, The University of Iowa, 4135E MERF, 375 Newton Rd, Iowa City, IA 52242, USAElizabeth A Faidley - Carver Family Center for Macular Degeneration, Department of Ophthalmology and Visual Sciences, The University of Iowa, 4135E MERF, 375 Newton Rd, Iowa City, IA 52242, USAHeather T Daggett - Carver Family Center for Macular Degeneration, Department of Ophthalmology and Visual Sciences, The University of Iowa, 4135E MERF, 375 Newton Rd, Iowa City, IA 52242, USACatherine Jomary - Clinical Neurosciences Division, The University of Southampton, UKAndrew J Lotery - Clinical Neurosciences Division, The University of Southampton, UKEdwin M Stone - Carver Family Center for Macular Degeneration, Department of Ophthalmology and Visual Sciences, The University of Iowa, 4135E MERF, 375 Newton Rd, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Experimental Eye Research, Vol.89(5), pp.767-773
- DOI
- 10.1016/j.exer.2009.07.001
- PMID
- 19607829
- PMCID
- PMC2757497
- NLM abbreviation
- Exp Eye Res
- ISSN
- 0014-4835
- eISSN
- 1096-0007
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 11/2009
- Academic Unit
- The University of Iowa Institute for Vision Research; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980038002771
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