Journal article
Localization of the ROMK potassium channel to the apical membrane of distal nephron in rat kidney
Kidney international, Vol.54(4), pp.1214-1223
10/1998
DOI: 10.1046/j.1523-1755.1998.00120.x
PMID: 9767537
Abstract
Localization of the ROMK potassium channel to the apical membrane of distal nephron in rat kidney.
The apical potassium (K+) channels mediate K+ recycling in thick ascending limb (TAL) and K+ secretion in cortical collecting duct (CCD). Recently, the cDNAs for a family of renal K+ channels, ROMK1, -2 and -3, were identified. Based on the biophysical properties and mRNA distribution, it is believed that these ROMK cDNAs encode the apical K+ channels of TAL and CCD. However, the information for cellular and subcellular localization of the ROMK proteins in these tubules is still not available.
Paraffin or frozen kidney sections from adult Sprague-Dawley rats were stained by polyclonal antibodies against the N- and C-terminal domain of ROMK. Immunoreactive staining was visualized by color development from horseradish peroxidase reaction. Membrane homogenates from kidney were analyzed by Western blot analysis.
The polyclonal antibodies against cytoplasmic epitope of ROMK recognized a ∼42 kD protein in the membrane homogenates from kidney, but not from liver. Staining by immunocytochemistry revealed that ROMK channels were localized to the apical membranes of the distal nephron in cortex and outer medulla, including thick ascending limb and collecting tubule. ROMK staining was absent in glomerulus, proximal tubule and inner medulla. Double staining of the tissue section with both ROMK-specific and H+-ATPase-specific antibodies revealed labeling of ROMK in the principal cells of the collecting tubules.
These results further strengthen the idea that ROMK channels play important roles in the recycling of K+ in TAL and the secretion of K+ in CCD.
Details
- Title: Subtitle
- Localization of the ROMK potassium channel to the apical membrane of distal nephron in rat kidney
- Creators
- Yukimasa Kohda - The University of Texas at DallasWei Ding - The University of Texas at DallasEm Phan - The University of Texas at DallasIhsan Housini - The University of Texas at DallasJin Wang - The University of Texas at DallasRobert A. Star - The University of Texas at DallasChou-Long Huang - Internal Medicine
- Resource Type
- Journal article
- Publication Details
- Kidney international, Vol.54(4), pp.1214-1223
- Publisher
- Elsevier Inc
- DOI
- 10.1046/j.1523-1755.1998.00120.x
- PMID
- 9767537
- ISSN
- 0085-2538
- eISSN
- 1523-1755
- Language
- English
- Date published
- 10/1998
- Academic Unit
- Nephrology; Internal Medicine
- Record Identifier
- 9984359818302771
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