Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle

Ling Yang; Danilo Licastro; Edda Cava; Nicola Veronese; Francesco Spelta; Wanda Rizza; Beatrice Bertozzi; Dennis T Villareal; Gökhan S Hotamisligil; John O Holloszy; Luigi Fontana

doi:10.1016/j.celrep.2015.12.042

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Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle

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Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle

Ling Yang, Danilo Licastro, Edda Cava, Nicola Veronese, Francesco Spelta, Wanda Rizza, Beatrice Bertozzi, Dennis T Villareal, Gökhan S Hotamisligil, John O Holloszy, …

Cell reports (Cambridge), Vol.14(3), pp.422-428

01/26/2016

DOI: 10.1016/j.celrep.2015.12.042

PMID: 26774472

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Abstract

Calorie restriction (CR) retards aging, acts as a hormetic intervention, and increases serum corticosterone and HSP70 expression in rodents. However, less is known regarding the effects of CR on these factors in humans. Serum cortisol and molecular chaperones and autophagic proteins were measured in the skeletal muscle of subjects on CR diets for 3–15 years and in control volunteers. Serum cortisol was higher in the CR group than in age-matched sedentary and endurance athlete groups (15.6 ± 4.6 ng/dl versus 12.3 ± 3.9 ng/dl and 11.2 ± 2.7 ng/dl, respectively; p ≤ 0.001). HSP70, Grp78, beclin-1, and LC3 mRNA and/or protein levels were higher in the skeletal muscle of the CR group compared to controls. Our data indicate that CR in humans is associated with sustained rises in serum cortisol, reduced inflammation, and increases in key molecular chaperones and autophagic mediators involved in cellular protein quality control and removal of dysfunctional proteins and organelles. [Display omitted] •Calorie restriction increases health-span and lifespan in model organisms•Little is known about the metabolic and molecular effects of CR in humans•CR inhibits inflammation in part by increasing serum cortisol concentration•CR elevates expression of genes and proteins that enhance protein quality control Yang et al. show that calorie restriction without malnutrition in humans inhibits inflammation, at least in part by elevating serum cortisol concentration, and increases chaperone and autophagy genes and proteins involved in protein quality control and organelle homeostasis in the removal of dysfunctional proteins and organelles from cell.

cortisol

calorie restriction

HSP70

autophagy

aldosterone

Details

Title: Subtitle: Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle
Creators: Ling Yang - Department of Genetics and Complex Diseases and Sabri Ülker Center, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA
Danilo Licastro - CBM Scrl—Genomics, Area Science Park, Basovizza, 34149 Trieste, Italy
Edda Cava - Division of Geriatrics and Nutritional Sciences and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO 63110, USA
Nicola Veronese - Division of Geriatrics and Nutritional Sciences and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO 63110, USA
Francesco Spelta - Division of Geriatrics and Nutritional Sciences and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO 63110, USA
Wanda Rizza - Division of Geriatrics and Nutritional Sciences and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO 63110, USA
Beatrice Bertozzi - Division of Geriatrics and Nutritional Sciences and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO 63110, USA
Dennis T Villareal - Division of Geriatrics and Nutritional Sciences and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO 63110, USA
Gökhan S Hotamisligil - Department of Genetics and Complex Diseases and Sabri Ülker Center, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA
John O Holloszy - Division of Geriatrics and Nutritional Sciences and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO 63110, USA
Luigi Fontana - Division of Geriatrics and Nutritional Sciences and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO 63110, USA
Resource Type: Journal article
Publication Details: Cell reports (Cambridge), Vol.14(3), pp.422-428
DOI: 10.1016/j.celrep.2015.12.042
PMID: 26774472
NLM abbreviation: Cell Rep
ISSN: 2211-1247
eISSN: 2211-1247
Publisher: Elsevier Inc
Language: English
Date published: 01/26/2016
Academic Unit: Molecular Physiology and Biophysics; Anatomy and Cell Biology
Record Identifier: 9984025476302771

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