Journal article
Long-lasting transcription in hippocampal area CA1 after contextual fear conditioning
Neurobiology of learning and memory, Vol.172, pp.107250-107250
07/2020
DOI: 10.1016/j.nlm.2020.107250
PMID: 32422278
Abstract
•Unbiased microarray analysis of gene transcription in hippocampal area CA1 7 days after contextual fear conditioning.•Fosl2 expression is upregulated for 7 days after conditioning.•Nfil3 expression is upregulated for 7 days after conditioning.
A fundamental question is how memory is stored for several weeks and even longer. A long-lasting increase in gene transcription has been suggested to mediate such long-term memory storage. Here, we used contextual fear conditioning in mice to search for lasting transcription that may contribute to long-term memory storage. Our study focussed on hippocampal area CA1, which has been suggested to have a role for at least one week in contextual fear memory. Using an unbiased microarray analysis followed by confirmatory quantitative real-time PCR, we identified an upregulation of two transcription factors, Fosl2 and Nfil3, which lasted for seven days after conditioning. To our knowledge these are the longest transcriptional changes ever detected in the hippocampus after contextual fear conditioning. Thus, our findings suggest novel transcriptional candidates for long-term memory storage.
Details
- Title: Subtitle
- Long-lasting transcription in hippocampal area CA1 after contextual fear conditioning
- Creators
- Keiko Mizuno - Maurice Wohl Clinical Neuroscience Institute, Department of Basic and Clinical Neuroscience, King’s College London, UKAaron R Jeffries - Biosciences, College of Life and Environmental Sciences, Geoffrey Pope, University of Exeter, Stocker Road, Exeter, EX4 4QD, UKTed Abel - Department of Molecular Physiology and Biophysics, Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USAK. Peter Giese - Maurice Wohl Clinical Neuroscience Institute, Department of Basic and Clinical Neuroscience, King’s College London, UK
- Resource Type
- Journal article
- Publication Details
- Neurobiology of learning and memory, Vol.172, pp.107250-107250
- DOI
- 10.1016/j.nlm.2020.107250
- PMID
- 32422278
- NLM abbreviation
- Neurobiol Learn Mem
- ISSN
- 1074-7427
- eISSN
- 1095-9564
- Publisher
- Elsevier Inc
- Grant note
- name: NIH, award: 2 RO1 MH087463-06
- Language
- English
- Date published
- 07/2020
- Academic Unit
- Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984070220402771
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