Journal article
Long-term Evaluation of Dentin Matrix Stability and Gelatinolytic Activity after Dentin Pretreatment with Caffeic Acid Phenethyl Ester
The journal of adhesive dentistry, Vol.22(3), pp.285-296
05/01/2020
DOI: 10.3290/j.jad.a44552
PMID: 32435769
Abstract
Purpose: To investigate the long-term effect of 0.05% or 0.1% caffeic acid phenethyl ester (CAPE) on dentin matrix stability and hybrid layer stability, using an etch-and-rinse (Adper Scotchbond Multipurpose/ASB) or a self-etch adhesive (Clearfil SE Bond/CSE).
Materials and Methods: Dentin matrix specimens were assigned to five groups: 0.05% or 0.1% CAPE, green tea (GT), and the controls distilled water (DW) and dimethyl sulfoxide (DMSO). Following immersion of specimens for 1 h, modulus of elasticity (ME) and dentin mass change (MG) were determined at 3 post-treatment time points: immediately afterwards and at 3 and 6 months. Collagen solubilization (CS) was estimated by hydroxyproline (HYP) quantification. Resin-dentin interfaces with both adhesives were assessed with in situ zymography tests to evaluate gelatinolytic activity ( GA). The dentin pretreatments were actively applied for 60 s. The sealing ability of aged resin-bonded slices was assessed by nanoleakage tests.
Results: GT increased immediate ME, which decreased significantly after 3 months (p < 0.0001). The CAPE groups did not differ from the control groups. GT provided a significant increase in dentin matrix mass after treatment (p < 0.0001). No significant differences regarding MG were observed for CAPE 0.1%, CAPE 0.05%, DW, and DMSO groups after 3 and 6 months. Cumulative HYP release revealed that CAPE groups and GT were statistically similar to DW and DMSO; the GT group exhibited statistically significantly less HYP release than did CAPE groups (p = 0.0073). Treatment with 0.05% or 0.1% CAPE presented lower GA when applied to ASB before acid conditioning (p < 0.05), but no differences were detected when the CAPE groups were applied to CSE. CAPE at 0.1% significantly reduced nanoleakage for CSE, and 0.05% CAPE with CSE presented levels of nanoleakage similar to those of the CSE control group.
Conclusion: CAPE at 0.05% or 0.01% did not influence ME, MG, or CS, but reduced GA when applied to ASB before acid conditioning. CAPE at 0.1% with CSE promoted adhesive layer integrity.
Details
- Title: Subtitle
- Long-term Evaluation of Dentin Matrix Stability and Gelatinolytic Activity after Dentin Pretreatment with Caffeic Acid Phenethyl Ester
- Creators
- Vivianne Oliveira Pedrosa - Sao Leopoldo Mandic Res Inst, Campinas, SP, BrazilEnrico Coser Bridi - Sao Leopoldo Mandic Res Inst, Campinas, SP, BrazilAriene Arcas Leme-Kraus - Univ Illinois, Coll Dent, Chicago, IL USAFabiana Mantovani Gomes Franca - Sao Leopoldo Mandic Res Inst, Campinas, SP, BrazilCecilia Pedroso Turssi - Sao Leopoldo Mandic Res Inst, Campinas, SP, BrazilFlavia Lucisano Botelho do Amaral - Sao Leopoldo Mandic Res Inst, Campinas, SP, BrazilLucas Novaes Teixeira - Sao Leopoldo Mandic Res Inst, Campinas, SP, BrazilElizabeth Ferreira Martinez - Sao Leopoldo Mandic Res Inst, Campinas, SP, BrazilAna Karina Bedran-Russo - Univ Illinois, Coll Dent, Chicago, IL USARoberta Tarkany Basting - Sao Leopoldo Mandic Res Inst, Campinas, SP, Brazil
- Resource Type
- Journal article
- Publication Details
- The journal of adhesive dentistry, Vol.22(3), pp.285-296
- Publisher
- Quintessence Publishing Co Inc
- DOI
- 10.3290/j.jad.a44552
- PMID
- 32435769
- ISSN
- 1461-5185
- eISSN
- 1757-9988
- Number of pages
- 12
- Language
- English
- Date published
- 05/01/2020
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984367753602771
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