Long-term bond strength and endogenous enzymatic activity of a chlorhexidine-containing commercially available adhesive

Tatjana Maravić; Allegra Comba; Sandra Ribeiro Cunha; Valeria Angeloni; Milena Cadenaro; Erika Visinitini; Chiara Ottavia Navarra; Stefano Salgarello; Lorenzo Breschi; Annalisa Mazzoni

doi:10.1016/j.jdent.2019.03.004

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Long-term bond strength and endogenous enzymatic activity of a chlorhexidine-containing commercially available adhesive

Journal article

Peer reviewed

Long-term bond strength and endogenous enzymatic activity of a chlorhexidine-containing commercially available adhesive

Tatjana Maravić, Allegra Comba, Sandra Ribeiro Cunha, Valeria Angeloni, Milena Cadenaro, Erika Visinitini, Chiara Ottavia Navarra, Stefano Salgarello, Lorenzo Breschi and Annalisa Mazzoni

Journal of dentistry, Vol.84, pp.60-66

05/2019

DOI: 10.1016/j.jdent.2019.03.004

PMID: 30876948

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Abstract

The aim of this study was to investigate, by the means of microtensile bond strength (μTBS) test, gelatin and in situ zymography, the influence of 0.2% CHX contained within a commercially available adhesive on long-term bond strength and endogenous enzymatic activity. Non-carious teeth were subjected to μTBS test (N = 15 for each group) and stressed until failure. μTBS was evaluated immediately and after 12-month storage in artificial saliva at 37 °C. Dentin powder was obtained from additional teeth (N = 7) for gelatin zymography, while for in situ zymography, 3 teeth for each group were selected. Gelatin and in situ zymography were performed in dentin powder and slices of dentin, respectively, to assess the ability of 0.2% CHX blended within the adhesive to inhibit endogenous enzymatic activity. μTBS bond strength was higher in the CHX-containing groups, immediately as well as after aging. The bond strength significantly decreased after 12-month aging. The activation of endogenous MMPs was found to be related to the presence of CHX within the adhesive system and the bonding strategy employed. Under this perspective 0.2% CHX blended within Peak Universal adhesive monomer seems to increase immediate bond strength, to preserve bond strength over time and to efficiently inhibit endogenous enzymatic activity in dentin. Hence, blending the CHX in low concentrations within the adhesive could be recommended as a feasible technique in every-day clinical practice. Using CHX-containing adhesives could be recommended due to several benefits: it seems to increase the longevity of the hybrid layer; the inhibitor appears to be efficiently delivered to the dentinal substrate and to inhibit endogenous enzymatic activity, without prolonging chair time.

Aging

Chlorhexidine

Dental bonding systems

Dentin

Hybrid Layer

Details

Title: Subtitle: Long-term bond strength and endogenous enzymatic activity of a chlorhexidine-containing commercially available adhesive
Creators: Tatjana Maravić - University of Bologna
Allegra Comba - University of Bologna
Sandra Ribeiro Cunha - Universidade de São Paulo
Valeria Angeloni - Private Practice, Via Martiri della Foce 20, 17031, Albenga, Italy
Milena Cadenaro - University of Trieste
Erika Visinitini - Ospedale di Cattinara
Chiara Ottavia Navarra - University of Trieste
Stefano Salgarello - University of Brescia
Lorenzo Breschi - University of Bologna
Annalisa Mazzoni - University of Bologna
Resource Type: Journal article
Publication Details: Journal of dentistry, Vol.84, pp.60-66
DOI: 10.1016/j.jdent.2019.03.004
PMID: 30876948
NLM abbreviation: J Dent
ISSN: 0300-5712
eISSN: 1879-176X
Publisher: Elsevier Ltd
Language: English
Date published: 05/2019
Academic Unit: Operative Dentistry
Record Identifier: 9984696654702771

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