Long-term effect of carbodiimide on dentin matrix and resin-dentin bonds

Ana Karina B BEDRAN-RUSSO; Cristina M. P VIDAL; Paulo H DOS SANTOS; Carina S CASTELLAN

doi:10.1002/jbm.b.31649

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Journal article

Long-term effect of carbodiimide on dentin matrix and resin-dentin bonds

Ana Karina B BEDRAN-RUSSO, Cristina M. P VIDAL, Paulo H DOS SANTOS and Carina S CASTELLAN

Journal of biomedical materials research. Part B, Applied biomaterials, Vol.94(1), pp.250-255

2010

DOI: 10.1002/jbm.b.31649

PMCID: PMC2882877

PMID: 20524201

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Abstract

Objectives: To characterize the interaction of 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide Hydrochloride (EDC) with dentin matrix and its effect on the resin-dentin bond. Methods: Changes to the stiffness of demineralized dentin fragments treated with EDC/N-hydroxysuccinimide (NHS) in different solutions were evaluated at different time points. The resistance against enzymatic degradation was indirectly evaluated by ultimate tensile strength (UTS) test of demineralized dentin treated or not with EDC/NHS and subjected to collagenase digestion. Short- and long-term evaluations of the strength of resin-dentin interfaces treated with EDC/NHS for 1 h were performed using microtensile bond strength (μTBS) test. All data (MPa) were individually analyzed using ANOVA and Tukey HSD tests (α = 0.05). Results: The different exposure times significantly increased the stiffness of dentin (p < 0.0001, control-5.15 and EDC/NHS-29.50), while no differences were observed among the different solutions of EDC/NHS (p = 0.063). Collagenase challenge did not affect the UTS values of EDC/NHS group (6.08) (p > 0.05), while complete degradation was observed for the control group (p = 0.0008, control-20.84 and EDC/NHS-43.15). EDC/NHS treatment did not significantly increase resin-dentin μTBS, but the values remained stable after 12 months water storage (p < 0.05). Conclusions: Biomimetic use of EDC/NHS to induce exogenous collagen cross-links resulted in increased mechanical properties and stability of dentin matrix and dentin-resin interfaces.

Technology. Biomaterials. Equipments

Biological and medical sciences

Medical sciences

Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases

Details

Title: Subtitle: Long-term effect of carbodiimide on dentin matrix and resin-dentin bonds
Creators: Ana Karina B BEDRAN-RUSSO - Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois 60612, United States
Cristina M. P VIDAL - Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois 60612, United States
Paulo H DOS SANTOS - Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois 60612, United States
Carina S CASTELLAN - Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois 60612, United States
Resource Type: Journal article
Publication Details: Journal of biomedical materials research. Part B, Applied biomaterials, Vol.94(1), pp.250-255
DOI: 10.1002/jbm.b.31649
PMID: 20524201
PMCID: PMC2882877
NLM abbreviation: J Biomed Mater Res B Appl Biomater
ISSN: 1552-4973
eISSN: 1552-4981
Publisher: Wiley-Blackwell; Hoboken, NJ
Language: English
Date published: 2010
Academic Unit: Operative Dentistry
Record Identifier: 9984065705302771

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