Long-term follow-up of blood donors with indeterminate human immunodeficiency virus type 1 results on Western blot

J. B JACKSON; M. R HANSON; G. M JOHNSON; T. G SPAHLINGER; H. F POLESKY; R. J BOWMAN

doi:10.1046/j.1537-2995.1995.35295125746.x

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Long-term follow-up of blood donors with indeterminate human immunodeficiency virus type 1 results on Western blot

Journal article

Peer reviewed

Long-term follow-up of blood donors with indeterminate human immunodeficiency virus type 1 results on Western blot

J. B JACKSON, M. R HANSON, G. M JOHNSON, T. G SPAHLINGER, H. F POLESKY and R. J BOWMAN

Transfusion (Philadelphia, Pa.), Vol.35(2), pp.98-102

1995

DOI: 10.1046/j.1537-2995.1995.35295125746.x

PMID: 7825219

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Abstract

BACKGROUND: At present, tens of thousands of United States blood donors who are at low risk for human immunodeficiency virus type 1 (HIV‐1) infection are indefinitely deferred. These persons are repeatably reactive for HIV‐1 antibody in enzyme immunoassay (EIA) and are indeterminate in Western blot. STUDY DESIGN AND METHODS: To determine the significance and persistence of anti‐HIV‐1 reactivity in plasma from volunteer blood donors with HIV‐1‐indeterminate Western blots, 66 donors were retested for HIV‐1 antibody by the same manufacturers' EIA and Western blot 5 to 7 years after the initial Western blot. In addition, donors' peripheral blood mononuclear cells were tested by polymerase chain reaction (PCR) for HIV‐1 DNA gag sequences. RESULTS: Thirty‐five (53%) of 66 donors were still repeatedly reactive for HIV‐1 on EIA and indeterminate on Western blot, 23 (35%) were negative on EIA and indeterminate on Western blot, 7 (11%) were negative in EIA and Western blot, and 1 (2%) was repeatedly reactive on EIA and negative on Western blot. Donors with persistently indeterminate Western blots had a band pattern nearly identical to that on the original Western blot. No donor was positive in Western blot, p24 antigen, or PCR testing. No donor had signs or symptoms of HIV‐1 infection. CONCLUSION: Long‐term follow‐up of Western blot‐indeterminate blood donors does not reveal evidence of HIV‐infection. A mechanism to return these donors to the donor pool should be considered.

Biological and medical sciences

Medical sciences

Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy

Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis

Transfusions. Complications. Transfusion reactions. Cell and gene therapy

Details

Title: Subtitle: Long-term follow-up of blood donors with indeterminate human immunodeficiency virus type 1 results on Western blot
Creators: J. B JACKSON - Case Western Reserve univ. school medicine, inst. pathology, Cleveland OH 44106, United States
M. R HANSON - Case Western Reserve univ. school medicine, inst. pathology, Cleveland OH 44106, United States
G. M JOHNSON - Case Western Reserve univ. school medicine, inst. pathology, Cleveland OH 44106, United States
T. G SPAHLINGER - Case Western Reserve univ. school medicine, inst. pathology, Cleveland OH 44106, United States
H. F POLESKY - Case Western Reserve univ. school medicine, inst. pathology, Cleveland OH 44106, United States
R. J BOWMAN - Case Western Reserve univ. school medicine, inst. pathology, Cleveland OH 44106, United States
Resource Type: Journal article
Publication Details: Transfusion (Philadelphia, Pa.), Vol.35(2), pp.98-102
Publisher: Blackwell Publishing; Oxford
DOI: 10.1046/j.1537-2995.1995.35295125746.x
PMID: 7825219
ISSN: 0041-1132
eISSN: 1537-2995
Language: English
Date published: 1995
Academic Unit: Pathology; VPMA - Administration
Record Identifier: 9984047981702771

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