Journal article
Longitudinal multi-omics analyses link gut microbiome dysbiosis with recurrent urinary tract infections in women
Nature microbiology, Vol.7(5), pp.630-639
05/01/2022
DOI: 10.1038/s41564-022-01107-x
PMID: 35505248
Abstract
Recurrent urinary tract infections (rUTIs) are a major health burden worldwide, with history of infection being a significant risk factor. While the gut is a known reservoir for uropathogenic bacteria, the role of the microbiota in rUTI remains unclear. We conducted a year-long study of women with (n = 15) and without (n = 16) history of rUTI, from whom we collected urine, blood and monthly faecal samples for metagenomic and transcriptomic interrogation. During the study 24 UTIs were reported, with additional samples collected during and after infection. The gut microbiome of individuals with a history of rUTI was significantly depleted in microbial richness and butyrate-producing bacteria compared with controls, reminiscent of other inflammatory conditions. However, Escherichia coli gut and bladder populations were comparable between cohorts in both relative abundance and phylogroup. Transcriptional analysis of peripheral blood mononuclear cells revealed expression profiles indicative of differential systemic immunity between cohorts. Altogether, these results suggest that rUTI susceptibility is in part mediated through the gut-bladder axis, comprising gut dysbiosis and differential immune response to bacterial bladder colonization, manifesting in symptoms.
Details
- Title: Subtitle
- Longitudinal multi-omics analyses link gut microbiome dysbiosis with recurrent urinary tract infections in women
- Creators
- Colin J Worby - Broad InstituteHenry L Schreiber IV - Washington University in St. Louis School of MedicineTimothy J Straub - Broad InstituteLucas R van Dijk - Broad InstituteRyan A Bronson - Broad InstituteBenjamin S Olson - Washington University in St. LouisJerome S Pinkner - Washington University in St. LouisChloe L P Obernuefemann - Washington University in St. LouisVanessa L Muñoz - Washington University in St. LouisAlexandra E Paharik - Washington University in St. LouisPhilippe N Azimzadeh - Washington University in St. LouisBruce J Walker - Applied Invention, Cambridge, MA, USAChristopher A Desjardins - Broad InstituteWen-Chi Chou - Broad InstituteKarla Bergeron - Washington University in St. LouisSinéad B Chapman - Broad InstituteAleksandra Klim - Washington University in St. LouisAbigail L Manson - Broad InstituteThomas J Hannan - Washington University in St. LouisThomas M Hooton - University of MiamiAndrew L Kau - Washington University in St. LouisH Henry Lai - Washington University in St. LouisKaren W Dodson - Washington University in St. LouisScott J Hultgren - Washington University in St. LouisAshlee M Earl - Broad Institute
- Resource Type
- Journal article
- Publication Details
- Nature microbiology, Vol.7(5), pp.630-639
- DOI
- 10.1038/s41564-022-01107-x
- PMID
- 35505248
- ISSN
- 2058-5276
- eISSN
- 2058-5276
- Grant note
- T32 GM139774 / NIGMS NIH HHS R01 AI165915 / NIAID NIH HHS U19 AI110818 / NIAID NIH HHS T32 GM007067 / NIGMS NIH HHS 2019083 / Doris Duke Charitable Foundation U01 AI095542 / NIAID NIH HHS R37 AI048689 / NIAID NIH HHS R01 DK121822 / NIDDK NIH HHS
- Language
- English
- Date published
- 05/01/2022
- Academic Unit
- Urology
- Record Identifier
- 9985244661902771