Loss of CD34 expression in aging human choriocapillaris endothelial cells

Elliott H Sohn; Miles J Flamme-Wiese; S Scott Whitmore; Kai Wang; Budd A Tucker; Robert F Mullins

doi:10.1371/journal.pone.0086538

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Loss of CD34 expression in aging human choriocapillaris endothelial cells

Journal article

Open access

Peer reviewed

Loss of CD34 expression in aging human choriocapillaris endothelial cells

Elliott H Sohn, Miles J Flamme-Wiese, S Scott Whitmore, Kai Wang, Budd A Tucker and Robert F Mullins

PloS one, Vol.9(1), pp.e86538-e86538

2014

DOI: 10.1371/journal.pone.0086538

PMCID: PMC3897719

PMID: 24466138

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Abstract

Structural and gene expression changes in the microvasculature of the human choroid occur during normal aging and age-related macular degeneration (AMD). In this study, we sought to determine the impact of aging and AMD on expression of the endothelial cell glycoprotein CD34. Sections from 58 human donor eyes were categorized as either young (under age 40), age-matched controls (> age 60 without AMD), or AMD affected (>age 60 with early AMD, geographic atrophy, or choroidal neovascularization). Dual labeling of sections with Ulex europaeus agglutinin-I lectin (UEA-I) and CD34 antibodies was performed, and the percentage of capillaries labeled with UEA-I but negative for anti-CD34 was determined. In addition, published databases of mouse and human retinal pigment epithelium-choroid were evaluated and CD34 expression compared between young and old eyes. Immunohistochemical studies revealed that while CD34 and UEA-I were colocalized in young eyes, there was variable loss of CD34 immunoreactivity in older donor eyes. While differences between normal aging and AMD were not significant, the percentage of CD34 negative capillaries in old eyes, compared to young eyes, was highly significant (p = 3.8×10(-6)). Endothelial cells in neovascular membranes were invariably CD34 positive. Published databases show either a significant decrease in Cd34 (mouse) or a trend toward decreased CD34 (human) in aging. These findings suggest that UEA-I and endogenous alkaline phosphatase activity are more consistent markers of aging endothelial cells in the choroid, and suggest a possible mechanism for the increased inflammatory milieu in the aging choroid.

Eye - metabolism

Retinal Pigment Epithelium - metabolism

Antigens, CD34 - metabolism

Choroid - metabolism

Endothelial Cells - metabolism

Humans

Middle Aged

Child, Preschool

Pigment Epithelium of Eye - metabolism

Young Adult

Macular Degeneration - metabolism

Choroidal Neovascularization - metabolism

Cellular Senescence

Adolescent

Aged, 80 and over

Adult

Aged

Capillaries - metabolism

Child

Aging - metabolism

Details

Title: Subtitle: Loss of CD34 expression in aging human choriocapillaris endothelial cells
Creators: Elliott H Sohn - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, United States of America ; Stephen A. Wynn Institute for Vision Research, The University of Iowa, Iowa City, Iowa, United States of America
Miles J Flamme-Wiese - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, United States of America ; Stephen A. Wynn Institute for Vision Research, The University of Iowa, Iowa City, Iowa, United States of America
S Scott Whitmore - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, United States of America ; Stephen A. Wynn Institute for Vision Research, The University of Iowa, Iowa City, Iowa, United States of America
Kai Wang - Stephen A. Wynn Institute for Vision Research, The University of Iowa, Iowa City, Iowa, United States of America ; Department of Biostatistics, The University of Iowa, Iowa City, Iowa, United States of America
Budd A Tucker - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, United States of America ; Stephen A. Wynn Institute for Vision Research, The University of Iowa, Iowa City, Iowa, United States of America
Robert F Mullins - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, United States of America ; Stephen A. Wynn Institute for Vision Research, The University of Iowa, Iowa City, Iowa, United States of America
Resource Type: Journal article
Publication Details: PloS one, Vol.9(1), pp.e86538-e86538
DOI: 10.1371/journal.pone.0086538
PMID: 24466138
PMCID: PMC3897719
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: United States
Grant note: T32 GM008629 / NIGMS NIH HHS EY017451 / NEI NIH HHS DP2 OD007483 / NIH HHS 1-DP2-OD007483-01 / NIH HHS
Language: English
Date published: 2014
Academic Unit: Biostatistics; Ophthalmology and Visual Sciences
Record Identifier: 9983980064202771

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