Loss of Drosophila Myb interrupts the progression of chromosome condensation

J Robert Manak; Hong Wen; Tran Van; Laura Andrejka; Joseph S Lipsick

doi:10.1038/ncb1580

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Loss of Drosophila Myb interrupts the progression of chromosome condensation

Journal article

Peer reviewed

Loss of Drosophila Myb interrupts the progression of chromosome condensation

J Robert Manak, Hong Wen, Tran Van, Laura Andrejka and Joseph S Lipsick

Nature cell biology, Vol.9(5), pp.581-587

05/2007

DOI: 10.1038/ncb1580

PMID: 17450131

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Abstract

Completion of chromosome condensation is required before segregation during the mitotic cell cycle to ensure the transmission of genetic material with high fidelity in a timely fashion. In many eukaryotes this condensation is regulated by phosphorylation of histone H3 on Ser 10 (H3S10). This phosphorylation normally begins in the late-replicating pericentric heterochromatin and then spreads to the earlier replicating euchromatin. Here, we show that these phases of condensation are genetically separable in that the absence of Drosophila Myb causes cells to arrest with H3S10 phosphorylation of heterochromatin but not euchromatin. In addition, we used mosaic analysis to demonstrate that although the Myb protein can be removed in a single cell cycle, the failure of chromosome condensation occurs only after many cell divisions in the absence of Myb protein. The Myb protein is normally located in euchromatic but not heterochromatic regions of the nucleus, suggesting that Myb may be essential for a modification of euchromatin that is required for the efficient spread of chromosome condensation.

Phosphorylation

Mutation

Cell Cycle Proteins - metabolism

Chromatin Assembly and Disassembly

Proto-Oncogene Proteins c-myb - genetics

Drosophila Proteins - metabolism

Serine - metabolism

Drosophila - physiology

Proto-Oncogene Proteins c-myb - metabolism

Euchromatin - metabolism

Proto-Oncogene Proteins c-myb - deficiency

Animals

Cell Nucleus - metabolism

Heterochromatin - metabolism

Mitosis - physiology

Drosophila Proteins - deficiency

Cell Cycle Proteins - genetics

Chromosomes - metabolism

Drosophila - embryology

Drosophila Proteins - genetics

Histones - metabolism

Larva - physiology

Drosophila - genetics

Details

Title: Subtitle: Loss of Drosophila Myb interrupts the progression of chromosome condensation
Creators: J Robert Manak - Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305-5342, USA
Hong Wen
Tran Van
Laura Andrejka
Joseph S Lipsick
Resource Type: Journal article
Publication Details: Nature cell biology, Vol.9(5), pp.581-587
DOI: 10.1038/ncb1580
PMID: 17450131
NLM abbreviation: Nat Cell Biol
ISSN: 1465-7392
eISSN: 1476-4679
Publisher: England
Grant note: R01 CA90307 / NCI NIH HHS
Language: English
Date published: 05/2007
Academic Unit: Stead Family Department of Pediatrics; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9983991990302771

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