Journal article
Loss of Flight and Associated Neuronal Rhythmicity in Inositol 1,4,5-Trisphosphate Receptor Mutants of Drosophila
The Journal of neuroscience, Vol.24(36), pp.7869-7878
09/08/2004
DOI: 10.1523/JNEUROSCI.0656-04.2004
PMCID: PMC1289272
PMID: 15356199
Abstract
Coordinated flight in winged insects requires rhythmic activity of the underlying neural circuit. Here, we show that
Drosophila
mutants for the inositol 1,4,5-trisphosphate (InsP
3
) receptor gene (
itpr
) are flightless. Electrophysiological recordings from thoracic indirect flight muscles show increased spontaneous firing accompanied by a loss of rhythmic flight activity patterns normally generated in response to a gentle puff of air. In contrast, climbing speed, the jump response, and electrical properties of the giant fiber pathway are normal, indicating that general motor coordination and neuronal excitability are much less sensitive to
itpr
mutations. All mutant phenotypes are rescued by expression of an
itpr
+
transgene in serotonin and dopamine neurons. Pharmacological and immunohistochemical experiments support the idea that the InsP
3
receptor functions to modulate flight specifically through serotonergic interneurons. InsP
3
receptor action appears to be important for normal development of the flight circuit and its central pattern generator.
Details
- Title: Subtitle
- Loss of Flight and Associated Neuronal Rhythmicity in Inositol 1,4,5-Trisphosphate Receptor Mutants of Drosophila
- Creators
- Santanu Banerjee - National Centre for Biological Sciences, Tata Institute of Fundamental Research, Gandhi Krishi Vigyan Kendra Campus, Bangalore 560065, India, andJisue Lee - Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242K Venkatesh - National Centre for Biological Sciences, Tata Institute of Fundamental Research, Gandhi Krishi Vigyan Kendra Campus, Bangalore 560065, India, andChun-Fang Wu - Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242Gaiti Hasan - National Centre for Biological Sciences, Tata Institute of Fundamental Research, Gandhi Krishi Vigyan Kendra Campus, Bangalore 560065, India, and
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.24(36), pp.7869-7878
- DOI
- 10.1523/JNEUROSCI.0656-04.2004
- PMID
- 15356199
- PMCID
- PMC1289272
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Language
- English
- Date published
- 09/08/2004
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070603602771
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