Loss of UBE3A from TH-expressing neurons suppresses GABA co-release and enhances VTA-NAc optical self-stimulation

Janet Berrios; Alice M Stamatakis; Pranish A Kantak; Zoe A McElligott; Matthew C Judson; Megumi Aita; Marie Rougie; Garret D Stuber; Benjamin D Philpot

doi:10.1038/ncomms10702

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Loss of UBE3A from TH-expressing neurons suppresses GABA co-release and enhances VTA-NAc optical self-stimulation

Journal article

Open access

Peer reviewed

Loss of UBE3A from TH-expressing neurons suppresses GABA co-release and enhances VTA-NAc optical self-stimulation

Janet Berrios, Alice M Stamatakis, Pranish A Kantak, Zoe A McElligott, Matthew C Judson, Megumi Aita, Marie Rougie, Garret D Stuber and Benjamin D Philpot

Nature communications, Vol.7(1), pp.10702-10702

02/12/2016

DOI: 10.1038/ncomms10702

PMCID: PMC4754338

PMID: 26869263

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Abstract

Motivated reward-seeking behaviours are governed by dopaminergic ventral tegmental area projections to the nucleus accumbens. In addition to dopamine, these mesoaccumbal terminals co-release other neurotransmitters including glutamate and GABA, whose roles in regulating motivated behaviours are currently being investigated. Here we demonstrate that loss of the E3-ubiquitin ligase, UBE3A, from tyrosine hydroxylase-expressing neurons impairs mesoaccumbal, non-canonical GABA co-release and enhances reward-seeking behaviour measured by optical self-stimulation.

Immunohistochemistry

Tyrosine 3-Monooxygenase - metabolism

Self Stimulation

Synaptic Transmission - genetics

Nucleus Accumbens - metabolism

Optogenetics

Ventral Tegmental Area - cytology

Neural Pathways

Behavior, Animal

Dopamine - secretion

Neurons - secretion

gamma-Aminobutyric Acid - secretion

Dopaminergic Neurons - secretion

Mice, Inbred C57BL

Reinforcement (Psychology)

In Situ Hybridization, Fluorescence

Mice, Knockout

Stereotaxic Techniques

Patch-Clamp Techniques

Animals

Ventral Tegmental Area - metabolism

Mice

Reward

Ubiquitin-Protein Ligases - genetics

Motivation - genetics

Details

Title: Subtitle: Loss of UBE3A from TH-expressing neurons suppresses GABA co-release and enhances VTA-NAc optical self-stimulation
Creators: Janet Berrios - Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA
Alice M Stamatakis - Inscopix Inc, Palo Alto, 94303 California, USA
Pranish A Kantak - Department of Psychiatry, University of North Carolina, Chapel Hill, 27599 North Carolina, USA
Zoe A McElligott - Bowles Center for Alcohol Studies, University of North Carolina, Chapel Hill, 27599 North Carolina, USA
Matthew C Judson - Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA
Megumi Aita - Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA
Marie Rougie - Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA
Garret D Stuber - Department of Psychiatry, University of North Carolina, Chapel Hill, 27599 North Carolina, USA
Benjamin D Philpot - Carolina Institute for Developmental Disabilities, University of North Carolina, Chapel Hill, 27599 North Carolina, USA
Resource Type: Journal article
Publication Details: Nature communications, Vol.7(1), pp.10702-10702
DOI: 10.1038/ncomms10702
PMID: 26869263
PMCID: PMC4754338
NLM abbreviation: Nat Commun
ISSN: 2041-1723
eISSN: 2041-1723
Publisher: England
Grant note: P30 NS045892 / NINDS NIH HHS R01 MH093372 / NIMH NIH HHS R01NS085093 / NINDS NIH HHS R01MH093372 / NIMH NIH HHS DA032750 / NIDA NIH HHS R01 DA032750 / NIDA NIH HHS HD079124 / NICHD NIH HHS U54 HD079124 / NICHD NIH HHS R01 NS085093 / NINDS NIH HHS
Language: English
Date published: 02/12/2016
Academic Unit: Periodontics
Record Identifier: 9984065814002771

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