Journal article
Loss of p15/Ink4b accompanies tumorigenesis triggered by complex DNA double-strand breaks
Carcinogenesis (New York), Vol.31(10), pp.1889-1896
10/2010
DOI: 10.1093/carcin/bgq153
PMCID: PMC2950935
PMID: 20663777
Abstract
DNA double-strand breaks (DSBs) are the most deleterious lesion inflicted by ionizing radiation. Although DSBs are potentially carcinogenic, it is not clear whether complex DSBs that are refractory to repair are more potently tumorigenic compared with simple breaks that can be rapidly repaired, correctly or incorrectly, by mammalian cells. We previously demonstrated that complex DSBs induced by high-linear energy transfer (LET) Fe ions are repaired slowly and incompletely, whereas those induced by low-LET gamma rays are repaired efficiently by mammalian cells. To determine whether Fe-induced DSBs are more potently tumorigenic than gamma ray-induced breaks, we irradiated 'sensitized' murine astrocytes that were deficient in Ink4a and Arf tumor suppressors and injected the surviving cells subcutaneously into nude mice. Using this model system, we find that Fe ions are potently tumorigenic, generating tumors with significantly higher frequency and shorter latency compared with tumors generated by gamma rays. Tumor formation by Fe-irradiated cells is accompanied by rampant genomic instability and multiple genomic changes, the most interesting of which is loss of the p15/Ink4b tumor suppressor due to deletion of a chromosomal region harboring the CDKN2A and CDKN2B loci. The additional loss of p15/Ink4b in tumors derived from cells that are already deficient in p16/Ink4a bolsters the hypothesis that p15 plays an important role in tumor suppression, especially in the absence of p16. Indeed, we find that reexpression of p15 in tumor-derived cells significantly attenuates the tumorigenic potential of these cells, indicating that p15 loss may be a critical event in tumorigenesis triggered by complex DSBs.
Details
- Title: Subtitle
- Loss of p15/Ink4b accompanies tumorigenesis triggered by complex DNA double-strand breaks
- Creators
- Cristel V Camacho - Department of Radiation Oncology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USABipasha MukherjeeBrian McEllinLiang-Hao DingBurong HuAmyn A HabibXian-Jin XieChaitanya S NirodiDebabrata SahaMichael D StoryAdayabalam S BalajeeRobert M BachooDavid A BoothmanSandeep Burma
- Resource Type
- Journal article
- Publication Details
- Carcinogenesis (New York), Vol.31(10), pp.1889-1896
- DOI
- 10.1093/carcin/bgq153
- PMID
- 20663777
- PMCID
- PMC2950935
- NLM abbreviation
- Carcinogenesis
- ISSN
- 0143-3334
- eISSN
- 1460-2180
- Publisher
- England
- Grant note
- P30 CA142543 / NCI NIH HHS NS062080 / NINDS NIH HHS T32CA124334 / NCI NIH HHS R01 CA139217 / NCI NIH HHS CA129364 / NCI NIH HHS RP100644 / PHS HHS CA122972 / NCI NIH HHS
- Language
- English
- Date published
- 10/2010
- Academic Unit
- Preventive and Community Dentistry; Biostatistics; Dental Research
- Record Identifier
- 9983917671602771
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