Loss of the CD5+ and CD45RAhi B cell subsets in alcoholics

R T Cook; T J Waldschmidt; B L Cook; D R Labrecque; K McLatchie

doi:10.1046/j.1365-2249.1996.d01-621.x

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Loss of the CD5+ and CD45RAhi B cell subsets in alcoholics

Journal article

Open access

Peer reviewed

Loss of the CD5+ and CD45RAhi B cell subsets in alcoholics

R T Cook, T J Waldschmidt, B L Cook, D R Labrecque and K McLatchie

Clinical and experimental immunology, Vol.103(2), pp.304-310

02/1996

DOI: 10.1046/j.1365-2249.1996.d01-621.x

PMCID: PMC2200354

PMID: 8565316

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Abstract

Chronic alcoholics are frequently immunodeficient, have polyclonal hypergammaglobulinaemia, and often have autoantibodies. Recent work in other diseases has shown that functional distinctions of possible relevance to autoimmunity and immunodeficiency can be found among the B cell subsets defined by differential expression of the surface markers CD5 and CD45RA. Therefore, we have evaluated the CD5, CD45RA B cell subsets of both chronic alcoholics without evidence of active liver disease (AWLD), and alcoholics admitted for acute alcoholic liver disease (ALD). Mean B cell numbers were normal in AWLD, but significantly reduced in ALD. Analysis of B cells by three-colour flow cytometry in 20 patients and 29 controls revealed a sharp decrease in the percentage of alcoholics' B cells which were CD5+, 37.6% versus 16.3%, P < 0.000 01; absolute CD5+ B cell numbers were similarly reduced (58.9 cells/microliters versus 20.9; P = 0.0012). In addition to the loss of CD5+ B cells, there was a reduction in the percentage of B cells which are CD5- CD45RAhi, leaving many patients with a B cell profile which was predominantly CD19+ CD5- CD45RAlo. This subset appears phenotypically similar to the IgM-producing CD5- CD45RAlo subset described by others, and may be enriched for autoantibody-producing cells. One outlier patient was an ALD with 61% of B cells which were CD5+, which also is a profile consistent with increased autoantibody production.

Leukocyte Common Antigens - immunology

B-Lymphocytes - immunology

Humans

Middle Aged

CD5 Antigens - immunology

Immunophenotyping

Cell Differentiation

B-Lymphocytes - pathology

Alcoholism - immunology

Lymphocyte Subsets - pathology

Lymphocyte Subsets - immunology

Details

Title: Subtitle: Loss of the CD5+ and CD45RAhi B cell subsets in alcoholics
Creators: R T Cook - Department of Pathology, Veterans Affairs Medical Centre, Iowa City, IA, USA
T J Waldschmidt
B L Cook
D R Labrecque
K McLatchie
Resource Type: Journal article
Publication Details: Clinical and experimental immunology, Vol.103(2), pp.304-310
Publisher: England
DOI: 10.1046/j.1365-2249.1996.d01-621.x
PMID: 8565316
PMCID: PMC2200354
ISSN: 0009-9104
eISSN: 1365-2249
Grant note: AA08402 / NIAAA NIH HHS AA09598 / NIAAA NIH HHS
Language: English
Date published: 02/1996
Academic Unit: Psychiatry; Gastroenterology and Hepatology; Pathology; Internal Medicine
Record Identifier: 9984047721502771

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