Journal article
Low Molecular Weight Disulfide Cross-Linking Peptides as Nonviral Gene Delivery Carriers
Bioconjugate chemistry, Vol.11(6), pp.901-909
11/01/2000
DOI: 10.1021/bc000056i
PMID: 11087340
Abstract
Cross-linking peptides have been developed by inserting multiple Cys residues into a 20 amino acid condensing peptide that polymerizes through disulfide bond formation when bound to DNA resulting in small, highly stable DNA condensates that mediate efficient in vitro gene transfer [McKenzie et al. (2000) J. Biol. Chem. 275, 9970-9977]. In the present study, a minimal peptide of four Lys and two terminal Cys residues was found to substitute for Cys-Trp-(Lys)17-Cys, resulting in DNA condensates with similar particle size and gene expression in HepG2 cells. Substitution of His for Lys residues resulted in an optimal peptide of Cys-His-(Lys)6-His-Cys that, in addition to the attributes described above, also provided buffering capacity to enhance in vitro gene expression in the absence of chloroquine. The reported structure-activity relationships systematically explore peptides with combinations of Lys, Cys, and His residues resulting in low molecular weight peptides with improved gene transfer properties.
Details
- Title: Subtitle
- Low Molecular Weight Disulfide Cross-Linking Peptides as Nonviral Gene Delivery Carriers
- Creators
- Donald L. McKenzie - University of MichiganElizabeth Smiley - University of MichiganKai Y. Kwok - University of MichiganKevin G. Rice - University of Michigan
- Resource Type
- Journal article
- Publication Details
- Bioconjugate chemistry, Vol.11(6), pp.901-909
- DOI
- 10.1021/bc000056i
- PMID
- 11087340
- NLM abbreviation
- Bioconjug Chem
- ISSN
- 1043-1802
- eISSN
- 1520-4812
- Language
- English
- Date published
- 11/01/2000
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984366039502771
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