Low concentrations of negatively charged sub-micron particles alter the microstructure of DPPC at the air–water interface

Amir M Farnoud; Jennifer Fiegel

doi:10.1016/j.colsurfa.2012.10.001

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Low concentrations of negatively charged sub-micron particles alter the microstructure of DPPC at the air–water interface

Journal article

Peer reviewed

Low concentrations of negatively charged sub-micron particles alter the microstructure of DPPC at the air–water interface

Amir M Farnoud and Jennifer Fiegel

Colloids and surfaces. A, Physicochemical and engineering aspects, Vol.415, pp.320-327

12/05/2012

DOI: 10.1016/j.colsurfa.2012.10.001

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Abstract

[Display omitted] ► Negatively charged polystyrene particles did not alter DPPC monolayer collapse. ► Particles increased the hysteresis area of DPPC surface pressure isotherm during cycling. ► Particles resulted in smaller but more numerous condensed DPPC domains. ► Particles were associated with the condensed DPPC domains. The function of dipalmitoyl phosphatidylcholine (DPPC) Langmuir monolayers was studied after exposure to various concentrations of 200nm carboxyl-modified polystyrene particles by a combination of surface pressure and surfactant microstructure studies. The presence of particles in the subphase at the lowest concentrations tested (10−5 to 10−4g/L) did not influence the π–A isotherms. However, at the highest concentration (10−3g/L), changes in the hysteresis areas of the isotherms were observed. The formation of LC domains during compression was significantly altered by the presence of the particles, resulting in the formation of smaller but more numerous domains. Fluorescence and atomic force microscopy images suggested that particles remained in the subphase but were closely associated with the condensed domains.

Air–water interface

DPPC

Fluorescence microscopy

Langmuir monolayer

Lipid domains

Surface pressure isotherm

Details

Title: Subtitle: Low concentrations of negatively charged sub-micron particles alter the microstructure of DPPC at the air–water interface
Creators: Amir M Farnoud - University of Iowa
Jennifer Fiegel - University of Iowa
Resource Type: Journal article
Publication Details: Colloids and surfaces. A, Physicochemical and engineering aspects, Vol.415, pp.320-327
Publisher: Elsevier B.V
DOI: 10.1016/j.colsurfa.2012.10.001
ISSN: 0927-7757
eISSN: 1873-4359
Language: English
Date published: 12/05/2012
Academic Unit: Chemical and Biochemical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
Record Identifier: 9984197194802771

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