Low-dose glyphosate exposure alters gut microbiota composition and modulates gut homeostasis

Peter C. Lehman; Nicole Cady; Sudeep Ghimire; Shailesh K. Shahi; Rachel L. Shrode; Hans-Joachim Lehmler; Ashutosh K. Mangalam

doi:10.1016/j.etap.2023.104149

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Low-dose glyphosate exposure alters gut microbiota composition and modulates gut homeostasis

Journal article

Open access

Peer reviewed

Low-dose glyphosate exposure alters gut microbiota composition and modulates gut homeostasis

Peter C. Lehman, Nicole Cady, Sudeep Ghimire, Shailesh K. Shahi, Rachel L. Shrode, Hans-Joachim Lehmler and Ashutosh K. Mangalam

Environmental toxicology and pharmacology, Vol.100, p.104149

06/2023

DOI: 10.1016/j.etap.2023.104149

PMCID: PMC10330715

PMID: 37196884

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Abstract

The widespread use of glyphosate, a broad-spectrum herbicide, has resulted in significant human exposure, and recent studies have challenged the notion that glyphosate is safe for humans. Although the link between disease states and glyphosate exposure is increasingly appreciated, the mechanistic links between glyphosate and its toxic effects on human health are poorly understood. Recent studies have suggested that glyphosate may cause toxicity through modulation of the gut microbiome, but evidence for glyphosate-induced gut dysbiosis and its effect on host physiology at doses approximating the U.S. Acceptable Daily Intake (ADI = 1.75 mg/kg body weight) is limited. Here, utilizing shotgun metagenomic sequencing of fecal samples from C57BL/6 J mice, we show that glyphosate exposure at doses approximating the U.S. ADI significantly impacts gut microbiota composition. These gut microbial alterations were associated with effects on gut homeostasis characterized by increased proinflammatory CD4+IL17A+ T cells and Lipocalin-2, a known marker of intestinal inflammation. •Low-dose glyphosate exposure modulates gut microbiota composition.•Depletion of Bifidobacterium and Lactobacillus after glyphosate-exposure.•Gut microbial alterations are associated with a pro-inflammatory response.

Inflammation

Glyphosate

Gut microbiota

Short-chain fatty acids

Details

Title: Subtitle: Low-dose glyphosate exposure alters gut microbiota composition and modulates gut homeostasis
Creators: Peter C. Lehman - University of Iowa
Nicole Cady - University of Michigan
Sudeep Ghimire - University of Iowa
Shailesh K. Shahi - University of Iowa
Rachel L. Shrode - University of Iowa
Hans-Joachim Lehmler - University of Iowa
Ashutosh K. Mangalam - University of Iowa
Resource Type: Journal article
Publication Details: Environmental toxicology and pharmacology, Vol.100, p.104149
DOI: 10.1016/j.etap.2023.104149
PMID: 37196884
PMCID: PMC10330715
NLM abbreviation: Environ Toxicol Pharmacol
ISSN: 1382-6689
eISSN: 1872-7077
Publisher: Elsevier B.V
Language: English
Date published: 06/2023
Academic Unit: Occupational and Environmental Health; Pathology; Iowa Neuroscience Institute
Record Identifier: 9984410798002771

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