Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish

Xueqian Wang; Haitao Hou; Kaida Song; Zhiqiang Zhang; Shuqiang Zhang; Ying Cao; Liming Chen; Qing Sang; Fang Lin; Hui Xu

doi:10.3389/fnmol.2018.00034

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Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish

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Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish

Xueqian Wang, Haitao Hou, Kaida Song, Zhiqiang Zhang, Shuqiang Zhang, Ying Cao, Liming Chen, Qing Sang, Fang Lin and Hui Xu

Frontiers in molecular neuroscience, Vol.11, pp.34-34

02/09/2018

DOI: 10.3389/fnmol.2018.00034

PMCID: PMC5812253

PMID: 29479307

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Abstract

LPA signaling plays important roles during cell migration and proliferation in normal and pathological conditions. However, its role during sensory organ development remains unknown. Here we show a LPA receptor Lpar2b is expressed in the posterior lateral line primordium (pLLP) and mechanosensory organs called neuromasts (NMs) in zebrafish embryos. Lpar2b loss-of-function significantly reduces the number of NMs and hair cells in the posterior lateral line (pLL). Further analysis reveals that Lpar2b regulates the patterning and tissue size of the pLLP. Interestingly, we show that knocking down a Hippo effector Yap1 phenocopies the result of Lpar2b depletion, and Lpar2b regulates the phosphorylation and activity of Yap1 in the pLLP. Importantly, a phosphorylation-resistant Yap1 rescues pLLP size and NM number in Lpar2b-depleted embryos. Our results indicate Lpar2b controls primordium size and NM number by regulating Yap1 activity in the lateral line system.

Neuroscience

hair cells

organ size

zebrafish

LPA

lateral line

neuromast

Details

Title: Subtitle: Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish
Creators: Xueqian Wang - Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University
Haitao Hou - Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University
Kaida Song - Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University
Zhiqiang Zhang - Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University
Shuqiang Zhang - Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University
Ying Cao - School of Life Science and Technology, Tongji University
Liming Chen - Biochemistry and Biological Product Institute, School of Life Sciences, Nanjing Normal University
Qing Sang - MOE Key Laboratory of Contemporary Anthropology and School of Life Sciences, Fudan University
Fang Lin - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa
Hui Xu - Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University
Resource Type: Journal article
Publication Details: Frontiers in molecular neuroscience, Vol.11, pp.34-34
DOI: 10.3389/fnmol.2018.00034
PMID: 29479307
PMCID: PMC5812253
NLM abbreviation: Front Mol Neurosci
ISSN: 1662-5099
eISSN: 1662-5099
Publisher: Frontiers Media S.A
Language: English
Date published: 02/09/2018
Academic Unit: Anatomy and Cell Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984025304302771

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