Lung structure phenotype variation in inbred mouse strains revealed through in vivo micro-CT imaging

Jacqueline Thiesse; Eman Namati; Jessica C Sieren; Amanda R Smith; Joseph M Reinhardt; Eric A Hoffman; Geoffrey McLennan

doi:10.1152/japplphysiol.01322.2009

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Lung structure phenotype variation in inbred mouse strains revealed through in vivo micro-CT imaging

Journal article

Open access

Peer reviewed

Lung structure phenotype variation in inbred mouse strains revealed through in vivo micro-CT imaging

Jacqueline Thiesse, Eman Namati, Jessica C Sieren, Amanda R Smith, Joseph M Reinhardt, Eric A Hoffman and Geoffrey McLennan

Journal of applied physiology (1985), Vol.109(6), pp.1960-1968

12/2010

DOI: 10.1152/japplphysiol.01322.2009

PMCID: PMC3006419

PMID: 20671036

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Abstract

Within pulmonary research, the development of mouse models has provided insight into disease development, progression, and treatment. Structural phenotypes of the lung in healthy inbred mouse strains are necessary for comparison to disease models. To date, progress in the assessment of lung function in these small animals using whole lung function tests has been made. However, assessment of in vivo lung structure of inbred mouse strains has yet to be well defined. Therefore, the link between the structure and function phenotypes is still unclear. With advancements in small animal imaging it is now possible to investigate lung structures such as the central and peripheral airways, whole lung, and lobar volumes of mice in vivo, through the use of micro-CT imaging. In this study, we performed in vivo micro-CT imaging of the C57BL/6, A/J, and BALB/c mouse strains using the intermittent iso-pressure breath hold (IIBH) technique. The resulting high-resolution images were used to extract lung structure phenotypes. The three-dimensional lobar structures and airways were defined and a meaningful mouse airway nomenclature was developed. In addition, using these techniques we have uncovered significant differences in the airway structures between inbred mouse strains in vivo.

3-dimensional mouse airway

airway nomenclature

micro-computed tomography

mouse lung phenotype

Details

Title: Subtitle: Lung structure phenotype variation in inbred mouse strains revealed through in vivo micro-CT imaging
Creators: Jacqueline Thiesse - University of Iowa
Eman Namati - Departments of 1Internal Medicine,
Jessica C Sieren - Departments of 1Internal Medicine,
Amanda R Smith - Departments of 1Internal Medicine,
Joseph M Reinhardt - Biomedical Engineering, and
Eric A Hoffman - University of Iowa
Geoffrey McLennan - University of Iowa
Resource Type: Journal article
Publication Details: Journal of applied physiology (1985), Vol.109(6), pp.1960-1968
DOI: 10.1152/japplphysiol.01322.2009
PMID: 20671036
PMCID: PMC3006419
NLM abbreviation: J Appl Physiol (1985)
ISSN: 8750-7587
eISSN: 1522-1601
Publisher: American Physiological Society
Language: English
Date published: 12/2010
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Radiology; Internal Medicine
Record Identifier: 9984197005402771

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