Journal article
Lutheran blood group glycoprotein and its newly characterized mouse homologue specifically bind α5 chain-containing human laminin with high affinity
Blood, Vol.97(1), pp.312-320
01/01/2001
DOI: 10.1182/blood.V97.1.312
Abstract
Abstract Lutheran blood group glycoproteins (Lu gps) are receptors for the extracellular matrix protein, laminin. Studies suggest that Lu gps may contribute to vaso-occlusion in sickle cell disease and it has recently been shown that sickle cells adhere to laminin isoforms containing the α5 chain (laminin 10/11). Laminin α5 is present in the subendothelium and is also a constituent of bone marrow sinusoids, suggesting a role for the Lu/laminin interaction in erythropoiesis. The objectives of the current study were to define more precisely the molecular interactions of the extracellular and intracellular regions of human Lu and to clone and characterize a mouse homologue. To this end, complementary DNA and genomic clones for the mouse homologue were sequenced and the mouse Lu gene mapped to a region on chromosome 7 with conserved synteny with human 19q13.2. Mouse and human Lu gps are highly conserved (72% identity) at the amino acid sequence level and both mouse and human Lu gps specifically bind laminin 10/11 with high affinity. Furthermore, the first 3, N-terminal, immunoglobulin superfamily domains of human Lu are critical for this interaction. The results indicated that the cytoplasmic domain of BRIC 221-labeled human Lu gp is linked with the spectrin-based skeleton, affording the speculation that this interaction may be critical for signal transduction. These results further support a role for Lu gps in sickle cell disease and indicate the utility of mouse models to explore the function of Lu gp-laminin 10/11 interaction in normal erythropoiesis and in sickle cell disease.
Details
- Title: Subtitle
- Lutheran blood group glycoprotein and its newly characterized mouse homologue specifically bind α5 chain-containing human laminin with high affinity
- Creators
- Stephen F Parsons - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomGloria Lee - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomFrances A Spring - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomThiebaut-Noel Willig - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomLuanne L Peters - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomJ. Aura Gimm - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomMichael J. A Tanner - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomNarla Mohandas - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomDavid J Anstee - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United KingdomJoel Anne Chasis - From the Bristol Institute for Transfusion Sciences, Bristol, United Kingdom; Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Berkeley CA; The Jackson Laboratory, Bar Harbor, ME; and University of Bristol, Bristol, United Kingdom
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.97(1), pp.312-320
- DOI
- 10.1182/blood.V97.1.312
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Language
- English
- Date published
- 01/01/2001
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984070766802771
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