Journal article
Lymphokine-activated killer (LAK) cells. I: Differential recovery of LAK, natural killer cells, and cytotoxic T lymphocytes after a sublethal dose of cyclophosphamide
The Journal of immunology (1950), Vol.137(7), pp.2380-2384
1986
DOI: 10.4049/jimmunol.137.7.2380
PMID: 3489763
Abstract
The effect of a sublethal dose of cyclophosphamide (CTX) administered in vivo on murine natural killer (NK) cells, lymphokine-activated killer (LAK) cells, and cytotoxic T lymphocytes (CTL) was examined. It was found that such a dose of CTX abolished all of the killer cell responses examined. The recovery of each response was then examined as a function of time. Allogeneic CTL responses were the first to recover and could be generated from spleen cells obtained 6 days after CTX administration. Fresh NK cell activity recovered by days 9 to 12. However, when spleen cells obtained 12 days after CTX administration were cultured with interleukin 2 (IL 2) for 5 days, they lost the ability to lyse YAC-1 cells, suggesting a distinction between "augmented" and "fresh" NK cell activity. H-2-restricted, trinitrophenyl-specific CTL activity could be generated by day 14 after CTX, provided the cultures were supplemented with exogenous IL 2. LAK could not be induced until day 21 post-CTX treatment. These data suggest that LAK precursors, CTL precursors, and NK cells are distinct cell populations. Additionally, this report introduces a model that may be useful in examining the differential contribution of NK and LAK to successful adoptive tumor immunotherapy.
Details
- Title: Subtitle
- Lymphokine-activated killer (LAK) cells. I: Differential recovery of LAK, natural killer cells, and cytotoxic T lymphocytes after a sublethal dose of cyclophosphamide
- Creators
- Zuhair K Ballas - Univ. Iowa, Iowa City veterans administration, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.137(7), pp.2380-2384
- DOI
- 10.4049/jimmunol.137.7.2380
- PMID
- 3489763
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- American Association of Immunologists
- Language
- English
- Date published
- 1986
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094647102771
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