Journal article
Lymphokine-activated killer (LAK) cells. III: Characterization of LAK precursors and susceptible target cells within the murine thymus
The Journal of immunology (1950), Vol.139(10), pp.3542-3549
1987
DOI: 10.4049/jimmunol.139.10.3542
PMID: 3500221
Abstract
In our search for a biologic role for lymphokine-activated killers (LAK), we examined their generation from murine thymocytes. Normal adult thymocytes were capable of generating LAK upon culture with relatively large doses (500 to 1000 U/ml) of interleukin 2. Normal thymocytes were fractionated into four subsets by virtue of their co-expression of the Lyt-2 and L3T4 markers: Lyt-2+ L3T4+ (2+4+); 2+4-; 2-4+; and 2-4-. None of these subsets had any natural killer activity. Upon examining the ability of these subsets to generate LAK, it was found that the 2-4- subset was the most potent and required the smallest relative amounts of interleukin 2. In addition to lysing tumor cells, thymus-derived LAK were capable of killing "fresh" 2+4+ thymocytes. Fresh 2+4-, 2-4+, 2-4-, and cortisone-resistant thymocytes were resistant to lysis by LAK. Upon mitogen stimulation, however, the cortisone-resistant thymocytes and 2+4- thymocytes became LAK-susceptible. These data demonstrate a possible mechanism for the elimination of the 2+4+ thymocyte subset which is generally believed to be a "dead-end" population. Moreover, these data suggest a possible biologic role for LAK in the process of thymocyte maturation and intrathymic selection.
Details
- Title: Subtitle
- Lymphokine-activated killer (LAK) cells. III: Characterization of LAK precursors and susceptible target cells within the murine thymus
- Creators
- Zuhair K Ballas - Iowa city veterans administration, Iowa City IA 52242, United StatesWendy Rasmussen - Iowa city veterans administration, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.139(10), pp.3542-3549
- Publisher
- American Association of Immunologists
- DOI
- 10.4049/jimmunol.139.10.3542
- PMID
- 3500221
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 1987
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094658802771
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