Journal article
Lymphokine-activated killer (LAK) cells. V: 8-Mercaptoguanosine as an IL-2-sparing agent in LAK generation
The Journal of immunology (1950), Vol.145(10), pp.3524-3531
1990
DOI: 10.4049/jimmunol.145.10.3524
PMID: 1700014
Abstract
Guanine ribonucleosides, substituted at the C8 position with either a bromine or a thiol group, have recently been shown to regulate several immunologic responses. We have previously shown that 8-mercaptoguanosine (8MG) can replace the requirement for cytokines in the generation of MHC-restricted CTL. In this paper, we examined the ability of 8MG to induce MHC-nonrestricted killer cells. We found that 8MG did not induce significant lytic activity from normal resting lymphocytes. However, 8MG was able to synergize with minimal amounts of IL-2 in inducing lytic activity similar to lymphokine-activated killers (LAK) in that both NK-sensitive and NK-resistant tumor cells were killed. Both the precursors and effectors of 8MG-LAK activity were similar to NK cells and were CD4- CD8- asialo-GM1+ NK1.1+. Similar to IL-2-induced LAK, 8MG-LAK were B220+. 8MG appeared to "stage" these precursor lymphocytes to become more responsive to IL-2 because optimal induction of 8MG-LAK required preincubation with 8MG before the addition of IL-2. This "staging" appeared to be due to the release of a "second signal" since it was readily inhibited by cyclosporine A. Anti-IFN-alpha beta was as efficient as cyclosporine A in inhibiting 8MG-LAK generation, whereas anti-IFN-gamma and anti-IL-1 did not exhibit significant inhibition. These findings suggest that 8MG can be of possible utility as an IL-2-sparing agent in LAK generation from NK cells.
Details
- Title: Subtitle
- Lymphokine-activated killer (LAK) cells. V: 8-Mercaptoguanosine as an IL-2-sparing agent in LAK generation
- Creators
- Robert A Thompson - Univ. Iowa, Iowa City veterans administration, dep. internal medicine, Iowa City IA 52242, United StatesZuhair K Ballas - Univ. Iowa, Iowa City veterans administration, dep. internal medicine, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.145(10), pp.3524-3531
- Publisher
- American Association of Immunologists
- DOI
- 10.4049/jimmunol.145.10.3524
- PMID
- 1700014
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 1990
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094771702771
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