Journal article
Lymphokine-activated killer (LAK) cells: VI. NK1.1 +, CD3 + LAK effectors are derived from CD4 −, CD8 −, NK1.1 − precursors
Cellular immunology, Vol.134(2), pp.296-313
1991
DOI: 10.1016/0008-8749(91)90304-T
PMID: 1827045
Abstract
Normal murine splenocytes cultured with IL2 for 6, but not 3, days contained an NK1.1
+, CD3
+ lytically active subset. These lymphocytes were not derived from NK1.1
+ precursors since NK1.1
+ cells, purified by flow cytometry, failed to express CD3, as determined by the 145-2C11 mAb, on their surface even after culture with IL2 for 6 days. Instead, the precursors of the NKl.1
+, CD3
+ effectors were contained in a B cell-depleted CD4
−, CD8
−, NK1.1
− splenic subset. Freshly obtained CD4
−, CD8
−, NK1.1
− splenocytes were mostly CD3
+, CD5
+, B220
−, had no spontaneous lytic activity against YAC-1, and were unable to mediate anti-CD3 directed lysis against FcR-bearing target cells. Culture of the CD4
−, CD8
−, NK1.1
− splenocytes with IL2, for 6 days, resulted in the development of NK1.1
+, CD3
+, B220
+ effectors 40% of which were CD5
dim and 20–25% of which expressed TCR-β8 as determined by the F23.1 mAb. The acquisition of NK1.1, B220, and lytic activity by this triple-negative subset was readily inhibited by cyclosporine A (CSA). On the other hand, CSA had no effect on the acquisition of B220 or lytic activity by NK1.1
+ precursors obtained by flow cytometry sorting. Moreover, all of the NK1.1
+ cells generated by IL2 culture of splenocytes obtained from mice depleted of NK1.1
+ lymphocytes (by
in vivo injection of anti-NK1.1 mAb) coexpressed CD3 on their surface and were thus distinct from classical NK cells. These findings demonstrate tjiat splenic NK cells do not express or acquire CD3; that the NK1.1
+, CD3
+ LAK effectors are derived from an NK1.1
− precursor; and that CSA is exquisitely selective in its inhibitory effect on LAK generation.
Details
- Title: Subtitle
- Lymphokine-activated killer (LAK) cells: VI. NK1.1 +, CD3 + LAK effectors are derived from CD4 −, CD8 −, NK1.1 − precursors
- Creators
- Zuhair K BallasWendy Rasmussen
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.134(2), pp.296-313
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0008-8749(91)90304-T
- PMID
- 1827045
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 1991
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094630002771
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