Journal article
Lysophosphatidic Acid (LPA) Is a Novel Extracellular Regulator of Cortical Neuroblast Morphology
Developmental biology, Vol.228(1), pp.6-18
12/01/2000
DOI: 10.1006/dbio.2000.9930
PMID: 11087622
Abstract
During cerebral cortical neurogenesis, neuroblasts in the ventricular zone (VZ) undergo a shape change termed “interkinetic nuclear migration” whereby cells alternate between fusiform and rounded morphologies. We previously identified lpA1, the first receptor gene for a signaling phospholipid called lysophosphatidic acid (LPA) and showed its enriched expression in the VZ. Here we report that LPA induces changes in neuroblast morphology from fusiform to round in primary culture, accompanied by nuclear movements, and formation of f-actin retraction fibers. These changes are mediated by the activation of the small GTPase, Rho. In explant cultures, where the cerebral cortical architecture remains intact, LPA not only induces cellular and nuclear rounding in the VZ, but also produces an accumulation of rounded nuclei at the ventricular surface. Consistent with a biological role for these responses, utilization of a sensitive and specific bioassay indicates that postmitotic neurons can produce extracellular LPA. These results implicate LPA as a novel factor in cortical neurogenesis and further implicate LPA as an extracellular signal from postmitotic neurons to proliferating neuroblasts.
Details
- Title: Subtitle
- Lysophosphatidic Acid (LPA) Is a Novel Extracellular Regulator of Cortical Neuroblast Morphology
- Creators
- Nobuyuki Fukushima - Department of Pharmacology, School of Medicine, University of California at San Diego, La Jolla, California, 92093-0636Joshua A Weiner - Neurosciences Program, School of Medicine, University of California at San Diego, La Jolla, California, 92093-0636Jerold Chun - Neurosciences Program, School of Medicine, University of California at San Diego, La Jolla, California, 92093-0636
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.228(1), pp.6-18
- DOI
- 10.1006/dbio.2000.9930
- PMID
- 11087622
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 12/01/2000
- Academic Unit
- Liberal Arts and Science Admin; Psychiatry; Iowa Neuroscience Institute; Biology
- Record Identifier
- 9983997988202771
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