Lysophosphatidic acid influences the morphology and motility of young, postmitotic cortical neurons

Nobuyuki Fukushima; Joshua A Weiner; Dhruv Kaushal; James J A Contos; Stevens K Rehen; Marcy A Kingsbury; Kyung Yong Kim; Jerold Chun

doi:10.1006/mcne.2002.1123

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Lysophosphatidic acid influences the morphology and motility of young, postmitotic cortical neurons

Journal article

Peer reviewed

Lysophosphatidic acid influences the morphology and motility of young, postmitotic cortical neurons

Nobuyuki Fukushima, Joshua A Weiner, Dhruv Kaushal, James J A Contos, Stevens K Rehen, Marcy A Kingsbury, Kyung Yong Kim and Jerold Chun

Molecular and cellular neurosciences, Vol.20(2), pp.271-282

06/2002

DOI: 10.1006/mcne.2002.1123

PMID: 12093159

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Abstract

Lysophosphatidic acid (LPA) is a bioactive lysophospholipid that produces process retraction and cell rounding through its cognate receptors in neuroblastoma cell lines. Although the expression profile of LPA receptors in developing brains suggests a role for LPA in central nervous system (CNS) development, how LPA influences the morphology of postmitotic CNS neurons remains to be determined. Here we have investigated the effects of exogenous LPA on the morphology of young, postmitotic neurons in primary culture. When treated with LPA, these neurons responded by not only retracting processes but also producing retraction fiber "caps" characterized by fine actin filaments emanating from a dense core. Retraction fiber caps gradually vanished due to the outward spread of regrowing membranes along the fibers, suggesting a role for caps as scaffolds for regrowth of retracted processes. Furthermore, LPA also affects neuronal migration in vitro and in vivo. Taken together, these results implicate LPA as an extracellular lipid signal affecting process outgrowth and migration of early postmitotic neurons during development.

Fetus

Pregnancy

Pseudopodia - ultrastructure

Microtubule-Associated Proteins - metabolism

Lysophospholipids - deficiency

Microtubule-Associated Proteins - drug effects

Cytochalasin D - pharmacology

RNA, Messenger - metabolism

Cerebral Cortex - cytology

Cell Movement - physiology

Cerebral Cortex - metabolism

Intracellular Membranes - ultrastructure

Tubulin - drug effects

Dose-Response Relationship, Drug

Pseudopodia - drug effects

Tubulin - metabolism

Actin Cytoskeleton - drug effects

Neurites - ultrastructure

Lysophospholipids - pharmacology

Female

Cell Differentiation - physiology

Neurites - drug effects

Gene Expression Regulation, Developmental - physiology

Actin Cytoskeleton - metabolism

Cell Size - drug effects

RNA, Messenger - genetics

Cells, Cultured

Cell Size - physiology

Neurites - metabolism

Mice, Knockout

Cell Movement - drug effects

Animals

Cerebral Cortex - embryology

Pseudopodia - metabolism

Signal Transduction - drug effects

Cell Differentiation - drug effects

Signal Transduction - physiology

Intracellular Membranes - drug effects

Mice

Mice, Inbred BALB C

Intracellular Membranes - metabolism

Polymers - metabolism

Details

Title: Subtitle: Lysophosphatidic acid influences the morphology and motility of young, postmitotic cortical neurons
Creators: Nobuyuki Fukushima - University of California San Diego
Joshua A Weiner - University of California San Diego
Dhruv Kaushal - University of California San Diego
James J A Contos - University of California San Diego
Stevens K Rehen - University of California San Diego
Marcy A Kingsbury - University of California San Diego
Kyung Yong Kim - University of California San Diego
Jerold Chun - University of California San Diego
Resource Type: Journal article
Publication Details: Molecular and cellular neurosciences, Vol.20(2), pp.271-282
DOI: 10.1006/mcne.2002.1123
PMID: 12093159
NLM abbreviation: Mol Cell Neurosci
ISSN: 1044-7431
eISSN: 1095-9327
Publisher: United States
Language: English
Date published: 06/2002
Academic Unit: Liberal Arts and Science Admin; Psychiatry; Iowa Neuroscience Institute; Biology
Record Identifier: 9983997989602771

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