Journal article
M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex
Nature cell biology, Vol.11(12), pp.1427-1432
12/2009
DOI: 10.1038/ncb1990
PMID: 19935652
Abstract
Cell-cell communication is essential for the development and homeostasis of multicellular organisms. Recently, a new type of cell-cell communication was discovered that is based on the formation of thin membranous nanotubes between remote cells. These long membrane tethers, termed tunneling nanotubes (TNTs), form an intercellular conduit and have been shown to enable the transport of various cellular components and signals. However, the molecular basis for TNT formation remains to be elucidated. Here we report that a mammalian protein, M-Sec, induces de novo formation of numerous membrane protrusions extending from the plasma membrane, some of which tether onto adjacent cells and subsequently form TNT-like structures. Depletion of M-Sec by RNA interference (RNAi) greatly reduced endogenous TNT formation as well as intercellular propagation of a calcium flux in a macrophage cell line. Furthermore, blockage of the interaction of M-Sec with Ral and the exocyst complex, which serves as a downstream effector of Ral, attenuated the formation of membrane nanotubes. Our results reveal that M-Sec functions as a key regulator of membrane nanotube formation through interaction with the Ral-exocyst pathway.
Details
- Title: Subtitle
- M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex
- Creators
- Hiroyuki Takatsu - Laboratory for Epithelial ImmunobiologySayaka Kawano - Laboratory for Epithelial ImmunobiologyHiroshi Watarai - Laboratory for Immune regulation, Research Center for Allergy and ImmunologyShunsuke Kimura - Laboratory for Epithelial ImmunobiologyCharles Yeaman - Department of Anatomy & Cell Biology, Carver College of Medicine, University of IowaHiroshi Ohno - Laboratory for Epithelial Immunobiology Department of Supramolecular Biology, Graduate School of Nanobioscience, Yokohama City UniversityMasumi Ohmae - Laboratory for Epithelial ImmunobiologyHiroshi Kitamura - Laboratory for ImmunogenomicsKoji Hase - Laboratory for Epithelial ImmunobiologyC. Clayton Hazelett - Department of Anatomy & Cell Biology, Carver College of Medicine, University of IowaMasatoshi Ito - Laboratory for Immunogenomics Department of Supramolecular Biology, Graduate School of Nanobioscience, Yokohama City University
- Resource Type
- Journal article
- Publication Details
- Nature cell biology, Vol.11(12), pp.1427-1432
- DOI
- 10.1038/ncb1990
- PMID
- 19935652
- ISSN
- 1465-7392
- eISSN
- 1476-4679
- Language
- English
- Date published
- 12/2009
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025443502771
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