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M1 macrophage-engineered vesicles have anti-cancer activity in ovarian cancer
Journal article   Open access   Peer reviewed

M1 macrophage-engineered vesicles have anti-cancer activity in ovarian cancer

Connie D. Cao, J. Robert McCorkle, Derek B. Allison, Donglin Yan, Kristen S. Hill, Lan Li, Rani Jayswal, David Schweer, Charles S. Dietrich, Frederick R. Ueland, …
Cancer nanotechnology, Vol.16(1), 35
07/22/2025
DOI: 10.1186/s12645-025-00337-y
PMCID: PMC12782215
PMID: 41522833
url
https://doi.org/10.1186/s12645-025-00337-yView
Published (Version of record) Open Access

Abstract

Background Ovarian cancer typically presents at an advanced stage, has a poor prognosis, and is a leading cause of cancer-related deaths in women. Extracellular vesicles (EVs) are cell membrane-derived nanoparticles that function in specific cell-to-cell communication and are under development as novel drug delivery vehicles and modulators of the tumor microenvironment. Artificial cell-derived vesicles (ACDVs) from M1 macrophages are able to repolarize macrophages from a M2 to a M1 phenotype and target tumor cells in in vitro studies. Results In this study, we generated engineered EVs (EEVs) by membrane disruption of M1 macrophages (MEVs) with and without cisplatin to generate cisplatin-loaded MEVs ( C -MEVs) and empty MEVs ( E -MEVs), which we tested in an ovarian cancer mouse xenograft model. E -MEVs and C -MEVs exhibited significantly less weight loss and equivalent activity to cisplatin, with improved activity over controls. Conclusions Further development of MEVs for the treatment of ovarian cancer is warranted.
Biochemistry Materials Science Nanotechnology Biomedical Engineering and Bioengineering Cancer Research Chemistry and Materials Science General

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