MERS versus Standard Surgical Approaches for Porcine Vocal Fold Scarring with Adipose Stem Cell Constructs

Joo Hyun Woo; Suzanne N King; Henry Hoffman; Seth Dailey; Sarah Wang; Michael B Christensen; Susan L Thibeault

doi:10.1177/0194599816645772

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MERS versus Standard Surgical Approaches for Porcine Vocal Fold Scarring with Adipose Stem Cell Constructs

Journal article

Open access

Peer reviewed

MERS versus Standard Surgical Approaches for Porcine Vocal Fold Scarring with Adipose Stem Cell Constructs

Joo Hyun Woo, Suzanne N King, Henry Hoffman, Seth Dailey, Sarah Wang, Michael B Christensen and Susan L Thibeault

Otolaryngology-head and neck surgery, Vol.155(4), pp.612-623

10/2016

DOI: 10.1177/0194599816645772

PMCID: PMC7032050

PMID: 27165681

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https://www.ncbi.nlm.nih.gov/pmc/articles/7032050View

Open Access

Abstract

Cells, scaffold, and surgical approaches are important for regeneration of the lamina propria of the scarred vocal fold (VF). Microendoscopy of Reinke's space (MERS) is a surgical approach used to access the lamina propria. The present study evaluated MERS in the treatment of VF scarring as compared with standardized approaches for the treatment of VF scarring with adipose stem cell constructs. Animal study. Academic center. VF injury was performed bilaterally to induce scarring in 20 pigs. Eight weeks after injury, pigs were classified into no treatment, minithyrotomy, VF injection, VF incision/dissection, and MERS. All groups (except control) were implanted with adipose stem cell and hyaluronan. Four weeks after treatment, histology for collagen, hyaluronan, and fibronectin; mRNA expression for α-smooth muscle actin, tumor growth factor β1, collagen 1α1, collagen 3α1, matrix metalloproteinase 2, basic fibroblast growth factor, and hepatocyte growth factor; and tissue rheology were evaluated. Differences were measured among surgical approaches for protein levels of collagen, hyaluronan, and fibronectin (P = .0133, P < .0001, and P = .0025, respectively). Fibroblast growth factor, collagen 1α1, and matrix metalloproteinase 2 transcript levels were different among treatment groups (P = .003, P = .0086, and P = .014, respectively), while no differences were measured for α-smooth muscle actin, tumor growth factor β1, hepatocyte growth factor, and collagen 3α1. Rheologically, significant differences were not measured between groups. MERS is a promising surgical approach for the treatment of VF scarring, optimizing the placement of implanted biomaterials.

Animals

Biomarkers - analysis

Cells, Cultured

Cicatrix - pathology

Cicatrix - surgery

Disease Models, Animal

Female

Fibrin Tissue Adhesive

Hydrogels - administration & dosage

Hydrogels - pharmacology

Immunoenzyme Techniques

Laryngoscopy

Real-Time Polymerase Chain Reaction

Stem Cell Transplantation

Swine

Vocal Cords - pathology

Vocal Cords - surgery

Details

Title: Subtitle: MERS versus Standard Surgical Approaches for Porcine Vocal Fold Scarring with Adipose Stem Cell Constructs
Creators: Joo Hyun Woo - Gachon University Gil Medical Center
Suzanne N King - University of Louisville
Henry Hoffman - University of Iowa
Seth Dailey - University of Wisconsin–Madison
Sarah Wang - University of Wisconsin–Madison
Michael B Christensen - University of Utah
Susan L Thibeault - University of Wisconsin–Madison
Resource Type: Journal article
Publication Details: Otolaryngology-head and neck surgery, Vol.155(4), pp.612-623
DOI: 10.1177/0194599816645772
PMID: 27165681
PMCID: PMC7032050
NLM abbreviation: Otolaryngol Head Neck Surg
ISSN: 0194-5998
eISSN: 1097-6817
Grant note: R01 DC004336 / NIDCD NIH HHS R01 DC013508 / NIDCD NIH HHS T32 DC009401 / NIDCD NIH HHS
Language: English
Date published: 10/2016
Academic Unit: Radiation Oncology; Otolaryngology
Record Identifier: 9984311443502771

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