Journal article
MR assessment of osteogenic differentiation in tissue-engineered constructs
Tissue engineering, Vol.12(4), pp.843-851
04/2006
DOI: 10.1089/ten.2006.12.843
PMID: 16674297
Abstract
Bone marrow stromal cells (MSC) are a promising source of osteoprogenitor cells for bone tissue engineering. However, the population of the osteoprogenitor cells and their differentiation potentials change with the gender, age, and health of the donor. Development of a noninvasive method to assess osteogenic progression is critical for successful bone tissue regeneration. High-resolution magnetic resonance imaging (MRI) (at 11.7 T, with spatial resolution of 62.5 x 62.5 microm in 500 microm slices) is used in the present study to monitor osteogenic differentiation of tissue-engineered constructs prepared by seeding human bone MSCs on gelatin sponge scaffolds. Quantitative measurements of the MR relaxation times (T1, T2) and the apparent diffusion coefficient (ADC) were performed for four successive weeks on control tissue constructs and constructs exposed to osteogenic differentiation medium. The T1 and T2 relaxation times and ADC were found to decrease as osteogenic progression proceeded in samples exposed to osteogenic differentiation medium. At week 4, the T1, T2, and ADC of TE constructs were 1.81 +/- 0.11 s, 19.5 +/- 11.02 ms, and 1.01 +/- 0.47 x 10(3) mm(2)/s, respectively, for osteogenic differentiated constructs, significantly different from control constructs 2.22 +/- 0.08 s, 50.39 +/- 5.57 ms, and 1.86 +/- 0.18 x 107(3) mm(2)/s (p < 0.05). The MR parameters were also highly correlated with the cell seeding densities and alkaline phosphatase (ALP) activities of the osteogenic constructs. In conclusion, periodic measurements of MR parameters (T1, T2, and ADC) provide a promising method for noninvasive monitoring of the status of tissue-engineered bone growth and differentiation.
Details
- Title: Subtitle
- MR assessment of osteogenic differentiation in tissue-engineered constructs
- Creators
- Ioana A Peptan - University of Illinois ChicagoLiu Hong - University of Illinois ChicagoHuihui Xu - University of Illinois ChicagoRichard L Magin - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Tissue engineering, Vol.12(4), pp.843-851
- DOI
- 10.1089/ten.2006.12.843
- PMID
- 16674297
- NLM abbreviation
- Tissue Eng
- ISSN
- 1076-3279
- eISSN
- 1557-8690
- Language
- English
- Date published
- 04/2006
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367748002771
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