MRIT, a novel death-effector domain-containing protein, interacts with caspases and BclXL and initiates cell death

David K.M Han; Preet M Chaudhary; Michael E Wright; Cynthia Friedman; Barbara J Trask; Rodney T Riedel; Dale G Baskin; Stephen M Schwartz; Leroy Hood

doi:10.1073/pnas.94.21.11333

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MRIT, a novel death-effector domain-containing protein, interacts with caspases and BclXL and initiates cell death

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MRIT, a novel death-effector domain-containing protein, interacts with caspases and BclXL and initiates cell death

David K.M Han, Preet M Chaudhary, Michael E Wright, Cynthia Friedman, Barbara J Trask, Rodney T Riedel, Dale G Baskin, Stephen M Schwartz and Leroy Hood

Proceedings of the National Academy of Sciences of the United States of America, Vol.94(21), pp.11333-11338

10/14/1997

DOI: 10.1073/pnas.94.21.11333

PMID: 9326610

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Abstract

Activation of the cascade of proteolytic caspases has been identified as the final common pathway of apoptosis in diverse biological systems. We have isolated a gene, termed MRIT, that possesses overall sequence homology to FLICE (MACH), a large prodomain caspase that links the aggregated complex of the death domain receptors of the tumor necrosis factor receptor family to downstream caspases. However, unlike FLICE, the C-terminal domain of MRIT lacks the caspase catalytic consensus sequence QAC(R/Q)G. Nonetheless MRIT activates caspase-dependent death. Using yeast two-hybrid assays, we demonstrate that MRIT associates with caspases possessing large and small prodomains (FLICE, and CPP32/YAMA), as well as with the adaptor molecule FADD. In addition, MRIT simultaneously and independently interacts with BclXL and FLICE in mammalian cells. Thus, MRIT is a mammalian protein that interacts simultaneously with both caspases and a Bcl-2 family member.

Genes

Proteins

Cellular biology

Details

Title: Subtitle: MRIT, a novel death-effector domain-containing protein, interacts with caspases and BclXL and initiates cell death
Creators: David K.M Han
Preet M Chaudhary
Michael E Wright - University of Iowa, Molecular Physiology and Biophysics
Cynthia Friedman
Barbara J Trask
Rodney T Riedel
Dale G Baskin
Stephen M Schwartz
Leroy Hood
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences of the United States of America, Vol.94(21), pp.11333-11338
DOI: 10.1073/pnas.94.21.11333
PMID: 9326610
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; Washington
Language: English
Date published: 10/14/1997
Academic Unit: Molecular Physiology and Biophysics
Record Identifier: 9983759100302771

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