Journal article
MULTICENTRE EVALUATION OF DISPOSABLE VISUAL MEASURING DEVICE TO ASSAY THEOPHYLLINE FROM CAPILLARY BLOOD SAMPLE
The Lancet (British edition), Vol.327(8474), pp.184-186
1986
DOI: 10.1016/S0140-6736(86)90655-0
Abstract
A device using enzyme immunochromatography to indicate visually theophylline concentration in a small capillary blood sample was evaluated for precision and accuracy at four hospitals. Preliminary studies indicated sensitivity as low as 2·5 μg/ml, and accuracy and precision matched those in test samples ranging from 5 to 30 μg/ml. Absence of theophylline was also reliably detected. Paired samples of capillary and venous blood from 214 patients were used to compare this method with four standard laboratory assays. Correlations with the standard assays were 0·93-0·97, slopes 1·00-1·27, and y intercepts - 1·91-0·46. The within-run coefficients of variation on twenty replicate patient sample analyses at the four hospitals were 5·1-9·7% and between-run coefficients of variation on a 15·9 μg/ml control 5·7-9·4%. This disposable device for theophylline monitoring, which can be done within 15 min at the patient's side without instruments, is sufficiently accurate to replace laboratory analysis for routine therapeutic drug monitoring.
Details
- Title: Subtitle
- MULTICENTRE EVALUATION OF DISPOSABLE VISUAL MEASURING DEVICE TO ASSAY THEOPHYLLINE FROM CAPILLARY BLOOD SAMPLE
- Creators
- Leigh M. Vaughan - University of IowaGary Milavetz - University of IowaElliot Ellis - Women & Children's Hospital of BuffaloStanley J Szefler - Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado, U.S.A.Kathleen Conboy - Women & Children's Hospital of BuffaloMiles M Weinberger - University of IowaStephen Tillson - Syntex Medical Diagnostics, Palo Alto, California;, United StatesJohn Jenne - Edward Hines, Jr. VA HospitalMatthew B Wiener - Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado, U.S.A.Tom Shaughnessy - Edward Hines, Jr. VA HospitalJulie Carrico - Syntex Medical Diagnostics, Palo Alto, California;, United States
- Resource Type
- Journal article
- Publication Details
- The Lancet (British edition), Vol.327(8474), pp.184-186
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/S0140-6736(86)90655-0
- ISSN
- 0140-6736
- eISSN
- 1474-547X
- Language
- English
- Date published
- 1986
- Academic Unit
- Pharmacy Practice and Science; Pharmacy; Stead Family Department of Pediatrics
- Record Identifier
- 9984366019602771
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