Journal article
MYC overexpression in inflammatory bowel disease-associated conventional dysplasia and association of subsequent low-grade dysplasia in follow-up biopsies
Pathology, research and practice, Vol.248, 154642
08/2023
DOI: 10.1016/j.prp.2023.154642
Abstract
Diagnosis of inflammatory bowel disease (IBD)-associated dysplastic lesions can be challenging. This study aims to evaluate MYC immunohistochemistry (IHC) as a potential biomarker for IBD-associated dysplasia and compare its effectiveness with p53 IHC.
The study cohort included resections from 12 IBD patients with carcinoma and concurrent conventional low-grade dysplasia (LGD), as well as biopsies from 21 patients with visible conventional LGD, which were followed up for 2 years with subsequent endoscopic examination. MYC and p53 IHC and MYC-FISH analysis were performed.
Sensitivity for LGD detection was 67% (8/12) and 50% (6/12) for MYC and p53, respectively, but the difference was not statistically significant (p = 0.2207). MYC and p53 overexpression were not always mutually exclusive, nor were they always present simultaneously. Patients who presented dysplasia in subsequent biopsies (7/21) were found to be more likely present with multiple LGD polyps and MYC-overexpressed LGD in the initial biopsies, compared to those without subsequent dysplasia (p < 0.05). These dysplastic lesions were commonly associated with chronic colitis (p = 0.0614). The distribution of LGD sites did not show a significant difference between patients with and without subsequent LGD. In MYC overexpressed cases, homogeneously strong nuclear expression was not identified in all dysplastic epithelial cells, and no MYC amplification was found in these cases by FISH.
MYC IHC can complement p53 IHC as an adjunct biomarker for diagnosing IBD-associated conventional LGD and can be used for the prediction of subsequent LGD in the follow-up biopsies combined with endoscopic features.
•Patients with multiple LGD and MYC-overexpressed LGD were likely to present dysplasia in subsequent biopsies.•MYC can complement p53 as an adjunct biomarker for diagnosing IBD-associated LGD.•MYC overexpression was not homogeneously strong in dysplastic cells and not associated with copy number amplification.
Details
- Title: Subtitle
- MYC overexpression in inflammatory bowel disease-associated conventional dysplasia and association of subsequent low-grade dysplasia in follow-up biopsies
- Creators
- Yuanxin Liang - Yale UniversityYansheng Hao - University of Rochester Medical CenterYiqin Xiong - University of IowaMinghao Zhong - Yale UniversityDhanpat Jain - Yale University
- Resource Type
- Journal article
- Publication Details
- Pathology, research and practice, Vol.248, 154642
- Publisher
- Elsevier GmbH
- DOI
- 10.1016/j.prp.2023.154642
- ISSN
- 0344-0338
- eISSN
- 1618-0631
- Language
- English
- Date published
- 08/2023
- Academic Unit
- Pathology
- Record Identifier
- 9984438958002771
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