Journal article
Macrophage Imaging Within Human Cerebral Aneurysms Wall Using Ferumoxytol-Enhanced MRI: A Pilot Study
Arteriosclerosis, thrombosis, and vascular biology, Vol.32(4), pp.1032-1038
2012
DOI: 10.1161/ATVBAHA.111.239871
PMCID: PMC3557844
PMID: 22328774
Abstract
Objective—
Macrophages play a critical role in cerebral aneurysm formation and rupture. The purpose of this study is to demonstrate the feasibility and optimal parameters of imaging macrophages within human cerebral aneurysm wall using ferumoxytol-enhanced MRI.
Methods and Results—
Nineteen unruptured aneurysms in 11 patients were imaged using T2*-GE–MRI sequence. Two protocols were used. Protocol A was an infusion of 2.5 mg/kg of ferumoxytol and imaging at day 0 and 1. Protocol B was an infusion of 5 mg/kg of ferumoxytol and imaging at day 0 and 3. All images were reviewed independently by 2 neuroradiologists to assess for ferumoxytol-associated loss of MRI signal intensity within aneurysm wall. Aneurysm tissue was harvested for histological analysis. Fifty percent (5/10) of aneurysms in protocol A showed ferumoxytol-associated signal changes in aneurysm walls compared to 78% (7/9) of aneurysms in protocol B. Aneurysm tissue harvested from patients infused with ferumoxytol stained positive for both CD68+, demonstrating macrophage infiltration, and Prussian blue, demonstrating uptake of iron particles. Tissue harvested from controls stained positive for CD68 but not Prussian blue.
Conclusion—
Imaging with T2*-GE–MRI at 72 hours postinfusion of 5 mg/kg of ferumoxytol establishes a valid and useful approximation of optimal dose and timing parameters for macrophages imaging within aneurysm wall. Further studies are needed to correlate these imaging findings with risk of intracranial aneurysm rupture.
Details
- Title: Subtitle
- Macrophage Imaging Within Human Cerebral Aneurysms Wall Using Ferumoxytol-Enhanced MRI: A Pilot Study
- Creators
- David M HASAN - Department of Neurosurgery, Carver College of Medicine, University of Iowa, Iowa City, IA, United StatesKelly B MAHANEY - Department of Neurosurgery, Carver College of Medicine, University of Iowa, Iowa City, IA, United StatesEdit DOSA - Department of Neurology Oregon Health and Science University, Portland, OR, United StatesEdward NEUWELT - Department of Neurology Oregon Health and Science University, Portland, OR, United StatesWilliam L YOUNG - Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, United StatesVincent A MAGNOTTA - Department of Radiology, Carver College of Medicine, University of Iowa, Iowa City, IA, United StatesDavid K KUNG - Department of Neurosurgery, Carver College of Medicine, University of Iowa, Iowa City, IA, United StatesMichael T LAWTON - Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, United StatesTomoki HASHIMOTO - Department of Anesthesia and Perioperative Care, University of California San Francisco, San Francisco, CA, United StatesH Richard Winn - Department of Neurosurgery , Hofstra University and Lenox Hill Hospital, New York City, NY, United StatesDavid SALONER - Department of Radiology , University of California San Francisco, San Francisco, CA, United StatesAlastair MARTIN - Department of Radiology , University of California San Francisco, San Francisco, CA, United StatesSeymur GAHRAMANOV - Department of Neurology Oregon Health and Science University, Portland, OR, United States
- Resource Type
- Journal article
- Publication Details
- Arteriosclerosis, thrombosis, and vascular biology, Vol.32(4), pp.1032-1038
- Publisher
- Lippincott Williams & Wilkins; Philadelphia, PA
- DOI
- 10.1161/ATVBAHA.111.239871
- PMID
- 22328774
- PMCID
- PMC3557844
- ISSN
- 1079-5642
- eISSN
- 1524-4636
- Language
- English
- Date published
- 2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Psychiatry; Iowa Neuroscience Institute; Neurosurgery; Otolaryngology
- Record Identifier
- 9984040226302771
Metrics
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