Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results

David Hasan; Nohra Chalouhi; Pascal Jabbour; Tomoki Hashimoto

doi:10.1186/1742-2094-9-222

Back

Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results

Journal article

Open access

Peer reviewed

Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results

David Hasan, Nohra Chalouhi, Pascal Jabbour and Tomoki Hashimoto

Journal of neuroinflammation, Vol.9(1), pp.222-222

09/21/2012

DOI: 10.1186/1742-2094-9-222

PMCID: PMC3488554

PMID: 22999528

Files and links (1)

url

https://doi.org/10.1186/1742-2094-9-222View

Published (Version of record) Open Access

Abstract

M1 and M2 cells are two major subsets of human macrophages that exert opposite effects on the inflammatory response. This study aims to investigate the role of macrophage M1/M2 imbalance and mast cells in the progression of human cerebral aneurysms to rupture. Ten patients with cerebral aneurysms (five ruptured and five unruptured) underwent microsurgical clipping. During the procedure, a segment of the aneurysm dome was resected and immunostained with monoclonal antibodies for M1 cells (anti-HLA DR), M2 cells (anti-CD 163), and mast cells (anti-tryptase clone AA). A segment of the superficial temporal artery (STA) was also removed and immunostained with monoclonal antibodies for M1, M2, and mast cells. All ten aneurysm tissues stained positive for M1, M2, and mast cells. M1 and M2 cells were present in equal proportions in unruptured aneurysms. This contrasted with a marked predominance of M1 over M2 cells in ruptured aneurysms (p = 0.045). Mast cells were also prominently upregulated in ruptured aneurysms (p = 0.001). Few M1 and M2 cells were present in STA samples. M1/M2 macrophages and mast cells are found in human cerebral aneurysms; however, M1 and mast cell expression seems to markedly increase in ruptured aneurysms. These findings suggest that macrophage M1/M2 imbalance and upregulation of mast cells may have a role in the progression of cerebral aneurysms to rupture.

Humans

Mast Cells - classification

Middle Aged

Temporal Arteries - pathology

Receptors, Cell Surface - metabolism

Male

Intracranial Aneurysm - complications

Aneurysm, Ruptured - complications

Antigens, CD - metabolism

Macrophages - metabolism

Antigens, Differentiation, Myelomonocytic - metabolism

Mast Cells - pathology

Up-Regulation - physiology

Mast Cells - metabolism

Adult

Female

Aged

Aneurysm, Ruptured - pathology

Intracranial Aneurysm - pathology

HLA-DR Antigens - metabolism

Details

Title: Subtitle: Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results
Creators: David Hasan - Department of Neurosurgery, Carver College of Medicine, University of Iowa, Iowa City, IA, USA. david-hasan@uiowa.edu
Nohra Chalouhi
Pascal Jabbour
Tomoki Hashimoto
Resource Type: Journal article
Publication Details: Journal of neuroinflammation, Vol.9(1), pp.222-222
DOI: 10.1186/1742-2094-9-222
PMID: 22999528
PMCID: PMC3488554
NLM abbreviation: J Neuroinflammation
ISSN: 1742-2094
eISSN: 1742-2094
Publisher: England
Grant note: P01 NS044155 / NINDS NIH HHS R03NS07922 / NINDS NIH HHS R01 NS055876 / NINDS NIH HHS
Language: English
Date published: 09/21/2012
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Neurosurgery; Otolaryngology
Record Identifier: 9984040003802771

Metrics

15 Record Views

144 Times Cited - Web of Science