Macrophage infiltration, but not apoptosis, is correlated with immune-mediated demyelination following murine infection with a neurotropic coronavirus

G F Wu; S Perlman

doi:10.1128/JVI.73.10.8771-8780.1999

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Macrophage infiltration, but not apoptosis, is correlated with immune-mediated demyelination following murine infection with a neurotropic coronavirus

Journal article

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Peer reviewed

Macrophage infiltration, but not apoptosis, is correlated with immune-mediated demyelination following murine infection with a neurotropic coronavirus

G F Wu and S Perlman

Journal of virology, Vol.73(10), pp.8771-8780

10/1999

DOI: 10.1128/JVI.73.10.8771-8780.1999

PMCID: PMC112898

PMID: 10482631

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https://europepmc.org/articles/pmc112898View

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Abstract

Mice infected with mouse hepatitis virus strain JHM (MHV-JHM) develop a chronic demyelinating encephalomyelitis that is in large part immune mediated. Potential mechanisms of immune activity were assessed using an adoptive transfer system. Mice deficient in recombinase-activating gene function (RAG1(-/-)), defective in B- and T-cell maturation, become persistently infected with MHV but do not develop demyelination. Adoptive transfer of splenocytes from mice immunized to MHV into RAG1(-/-) mice infected with an attenuated strain of the virus results in the rapid and progressive development of demyelination. Most striking, adoptive transfer resulted, within 5 to 6 days, in extensive recruitment of activated macrophages/microglia to sites of demyelination within the spinal cord. Clearance of virus antigen occurred preferentially from the gray matter of the spinal cord. Apoptotic cells were identified in both the gray and white matter of the central nervous system (CNS) from RAG1(-/-) mice before and after adoptive transfer, with a moderate increase in number, but not distribution, of apoptotic cells following the development of demyelination. These results suggest that apoptosis following MHV-JHM infection of the murine CNS is not sufficient to cause demyelination. These results, showing that macrophage recruitment and myelin destruction occur rapidly after immune reconstitution of RAG(-/-) mice, suggest that this will be a useful system for investigating MHV-induced demyelination.

Antigens, Viral - immunology

Animals

Macrophage Activation - immunology

Demyelinating Diseases - virology

Coronavirus Infections - pathology

Demyelinating Diseases - immunology

Mice

Coronavirus Infections - immunology

Murine hepatitis virus

Apoptosis

Cell Movement

Details

Title: Subtitle: Macrophage infiltration, but not apoptosis, is correlated with immune-mediated demyelination following murine infection with a neurotropic coronavirus
Creators: G F Wu - Program in Neuroscience, University of Iowa, Iowa City, Iowa 52242, USA
S Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.73(10), pp.8771-8780
DOI: 10.1128/JVI.73.10.8771-8780.1999
PMID: 10482631
PMCID: PMC112898
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: F30 MH012066 / NIMH NIH HHS NS 36592 / NINDS NIH HHS MH12066-02 / NIMH NIH HHS R01 NS036592 / NINDS NIH HHS
Language: English
Date published: 10/1999
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777351402771

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