Journal article
Macropinocytosis and cytoskeleton contribute to dendritic cell-mediated HIV-1 transmission to CD4 + T cells
Virology (New York, N.Y.), Vol.381(1), pp.143-154
2008
DOI: 10.1016/j.virol.2008.08.028
PMCID: PMC2575140
PMID: 18804253
Abstract
Dendritic cells (DCs) are among the first immune cells to encounter HIV-1 at the initial infection. DCs efficiently transfer HIV-1 to CD4
+ T cells via infectious or virological synapses formed between DCs and T cells. Retroviruses exploit the cytoskeletal network to facilitate viral infection and dissemination; however, the role of the cytoskeleton in DC-mediated HIV-1 transmission is unknown. Here, we report that intact cytoskeleton is essential for DC-mediated HIV-1 transmission to CD4
+ T cells. We found that macropinocytosis of HIV-1 contributes to DC-mediated HIV-1 endocytosis and transmission. Blocking HIV-1 macropinocytosis and disrupting actin or microtubules in DCs with specific inhibitors significantly prevented DC-mediated HIV-1
trans-infection of CD4
+ T cells. Altered HIV-1 trafficking and impaired formation of virological synapses primarily accounted for the inhibition of viral transmission by cytoskeletal inhibitors. Our results provide new insights into the mechanisms underlying DC-mediated HIV-1 transmission to CD4
+ T cells via the cytoskeletal network.
Details
- Title: Subtitle
- Macropinocytosis and cytoskeleton contribute to dendritic cell-mediated HIV-1 transmission to CD4 + T cells
- Creators
- Jian-Hua Wang - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USAClive Wells - Electron Microscopy Core Facility, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USALi Wu - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.381(1), pp.143-154
- DOI
- 10.1016/j.virol.2008.08.028
- PMID
- 18804253
- PMCID
- PMC2575140
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: AI068493; DOI: 10.13039/100009323, name: Campbell Foundation, award: 1S10RR022412-01
- Language
- English
- Date published
- 2008
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001223802771
Metrics
17 Record Views