Journal article
Macular and peripheral distribution of ICAM-1 in the human choriocapillaris and retina
Molecular vision, Vol.12(24-27), pp.224-235
03/30/2006
PMID: 16604055
Abstract
In order to understand the extent of choriocapillary endothelial cell activation in different topographic regions of the eye, we sought to compare the localization of intercellular adhesion molecule-1 (ICAM-1) in macular and peripheral regions of human eyes. Sections of sucrose-embedded human donor eyes that included the macula and ora serrata were evaluated for ICAM-1 and ICAM-2 immunoreactivity with monoclonal and polyclonal antibodies. Patterns of ICAM-1 labeling in peripheral and macular regions were examined in 20 eyes. Morphometric analyses of anti-ICAM-1 labeling intensity in the choriocapillaris were performed using ImageJ software on a series of macular and extramacular punches from nine eyes. Quantitative PCR analysis for ICAM-1 mRNA was performed on the RPE-choroid from the same regions from six of the same eyes, and Western blots of samples treated or untreated with N-glycosidase were performed to compare retinal and choroidal ICAM-1. ICAM-1 labeling of the choriocapillaris was typically more intense in the macula than in the peripheral choroid in human donor eyes (14/20). ICAM-2 was also detected in the choriocapillaris and retinal vessels. Morphometric measurements confirmed a significant macular-extramacular difference in ICAM-1 in six of nine eyes (p<0.05), with 1 of 9 eyes showing the opposite pattern. This pattern was not noted for endogenous alkaline phosphatase or ICAM-2. The opposite pattern was noted in the external limiting membrane (ELM), which exhibited more intense ICAM-1 labeling in the far periphery than in the macula. On Western blots, choroidal ICAM-1 exhibited a greater molecular weight than the retinal form, with most of the apparent weight difference due to N-linked carbohydrate chains. The regional differences in ICAM-1 distribution in the choriocapillaris may indicate that this region is subject to increased leukocyte trafficking. In view of the role of inflammatory processes in age-related macular degeneration (AMD), we propose that the higher level of ICAM-1 protein in the macular choriocapillaris may impart greater susceptibility of the macula to immune cell-mediated damage in AMD.
Details
- Title: Subtitle
- Macular and peripheral distribution of ICAM-1 in the human choriocapillaris and retina
- Creators
- Robert F Mullins - Center for Macular Degeneration, Department of Ophthalmology and Visual Science, The University of Iowa Carver College of Medicine, Iowa City, IA, USA. robert-mullins@uiowa.eduJessica M SkeieElizabeth A MaloneMarkus H Kuehn
- Resource Type
- Journal article
- Publication Details
- Molecular vision, Vol.12(24-27), pp.224-235
- Publisher
- United States
- PMID
- 16604055
- ISSN
- 1090-0535
- eISSN
- 1090-0535
- Grant note
- F32 EY022280 / NEI NIH HHS R03 EY14563 / NEI NIH HHS
- Language
- English
- Date published
- 03/30/2006
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9983980010902771
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