Journal article
Magnetization transfer imaging provides a quantitative measure of chondrogenic differentiation and tissue development
Tissue engineering. Part C, Methods, Vol.16(6), pp.1407-1415
12/2010
DOI: 10.1089/ten.tec.2009.0777
PMCID: PMC2988628
PMID: 20373975
Abstract
The goal of the present investigation was to test whether quantitative magnetization transfer imaging can be used as a noninvasive evaluation method for engineered cartilage. In this work, we used magnetic resonance imaging (MRI) to monitor the chondrogenesis of stem-cell-based engineered tissue over a 3-week period by measuring on a pixel-by-pixel basis the relaxation times (T₁ and T₂), the apparent diffusion coefficient, and the magnetization transfer parameters: bound proton fraction and cross-relaxation rate (k). Tissue-engineered constructs for generating cartilage were created by seeding mesenchymal stem cells in a gelatin sponge. Every 7 days, tissue samples were analyzed using MRI, histological, and biochemical methods. The MRI measurements were verified by histological analysis, and the imaging data were correlated with biochemical analysis of the developing cartilage matrix for glycosaminoglycan content. The MRI analysis for bound proton fraction and k showed a statistically significant increase that was correlated with the increase of glycosaminoglycan (R = 0.96 and 0.87, respectively, p < 0.05), whereas T₁, T₂, and apparent diffusion coefficient results did not show any significant changes over the 3-week measurement period.
Details
- Title: Subtitle
- Magnetization transfer imaging provides a quantitative measure of chondrogenic differentiation and tissue development
- Creators
- Weiguo Li - University of Illinois ChicagoLiu Hong - University of IowaLiping Hu - Northwestern MedicineRichard L Magin - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Tissue engineering. Part C, Methods, Vol.16(6), pp.1407-1415
- DOI
- 10.1089/ten.tec.2009.0777
- PMID
- 20373975
- PMCID
- PMC2988628
- NLM abbreviation
- Tissue Eng Part C Methods
- ISSN
- 1937-3384
- eISSN
- 1937-3392
- Grant note
- R01 EB007537 / NIBIB NIH HHS R01 EB007537-02 / NIBIB NIH HHS R01-EB007537 / NIBIB NIH HHS
- Language
- English
- Date published
- 12/2010
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367633902771
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