Main chain and side chain dynamics of oxidized flavodoxin from Cyanobacterium anabaena

Weixia Liu; Peter F Flynn; Ernesto J Fuentes; James K Kranz; Margaret  McCormick; A. Joshua Wand

doi:10.1021/bi011073d

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Main chain and side chain dynamics of oxidized flavodoxin from Cyanobacterium anabaena

Journal article

Peer reviewed

Main chain and side chain dynamics of oxidized flavodoxin from Cyanobacterium anabaena

Weixia Liu, Peter F Flynn, Ernesto J Fuentes, James K Kranz, Margaret McCormick and A. Joshua Wand

Biochemistry (Easton), Vol.40(49), pp.14744-14753

12/11/2001

DOI: 10.1021/bi011073d

PMID: 11732893

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Abstract

Oxidized flavodoxin from Cyanobacterium anabaena PCC 7119 is used as a model system to investigate the fast internal dynamics of a flavin-bearing protein. Virtually complete backbone and side chain resonance NMR assignments of an oxidized flavodoxin point mutant (C55A) have been determined. Backbone and side chain dynamics in flavodoxin (C55A) were investigated using (15)N amide and deuterium methyl NMR relaxation methods. The squared generalized order parameters (S(NH)(2)) for backbone amide N-H bonds are found to be uniformly high (<S(NH)(2)> approximately 0.923 over 109 residues in regular secondary structure), indicating considerable restriction of motion in the backbone of the protein. In contrast, methyl-bearing side chains are considerably heterogeneous in their amplitude of motion, as indicated by obtained symmetry axis squared generalized order parameters (S(axis)(2)). However, in comparison to nonprosthetic group-bearing proteins studied with these NMR relaxation methods, the side chains of oxidized flavodoxin are unusually rigid.

Protein Structure, Tertiary

Recombinant Proteins - metabolism

Flavodoxin - metabolism

Flavodoxin - genetics

Oxidation-Reduction

Cyanobacteria - chemistry

Bacterial Proteins - chemistry

Bacterial Proteins - genetics

Cyanobacteria - metabolism

Flavodoxin - chemistry

Models, Molecular

Recombinant Proteins - chemistry

Nuclear Magnetic Resonance, Biomolecular

Bacterial Proteins - metabolism

Protein Conformation

Details

Title: Subtitle: Main chain and side chain dynamics of oxidized flavodoxin from Cyanobacterium anabaena
Creators: Weixia Liu - The Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Peter F Flynn
Ernesto J Fuentes
James K Kranz
Margaret McCormick
A. Joshua Wand
Resource Type: Journal article
Publication Details: Biochemistry (Easton), Vol.40(49), pp.14744-14753
Publisher: United States
DOI: 10.1021/bi011073d
PMID: 11732893
ISSN: 0006-2960
eISSN: 1520-4995
Grant note: GM35940 / NIGMS NIH HHS
Language: English
Date published: 12/11/2001
Academic Unit: Biochemistry and Molecular Biology
Record Identifier: 9984024533302771

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