Journal article
Maintenance of homeostatic plasticity at the Drosophila neuromuscular synapse requires continuous IP3-directed signaling
eLife, Vol.8, e39643
06/10/2019
DOI: 10.7554/eLife.39643
PMCID: PMC6557630
PMID: 31180325
Abstract
Synapses and circuits rely on neuroplasticity to adjust output and meet physiological needs. Forms of homeostatic synaptic plasticity impart stability at synapses by countering destabilizing perturbations. The Drosophila melanogaster larval neuromuscular junction (NMJ) is a model synapse with robust expression of homeostatic plasticity. At the NMJ, a homeostatic system detects impaired postsynaptic sensitivity to neurotransmitter and activates a retrograde signal that restores synaptic function by adjusting neurotransmitter release. This process has been separated into temporally distinct phases, induction and maintenance. One prevailing hypothesis is that a shared mechanism governs both phases. Here, we show the two phases are separable. Combining genetics, pharmacology, and electrophysiology, we find that a signaling system consisting of PLCβ, inositol triphosphate (IP3), IP3 receptors, and Ryanodine receptors is required only for the maintenance of homeostatic plasticity. We also find that the NMJ is capable of inducing homeostatic signaling even when its sustained maintenance process is absent.Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (<xref ref-type="decision-letter" rid="SA1">see decision letter ).
Details
- Title: Subtitle
- Maintenance of homeostatic plasticity at the Drosophila neuromuscular synapse requires continuous IP3-directed signaling
- Creators
- Thomas D James - Department of Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, United States, Interdisciplinary Graduate Program in Neuroscience, University of Iowa, Iowa City, United StatesDanielle J Zwiefelhofer - Department of Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, United StatesC Andrew Frank - Department of Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, United States, Interdisciplinary Programs in Neuroscience, Genetics and Molecular Medicine, University of Iowa, Iowa City, United States
- Resource Type
- Journal article
- Publication Details
- eLife, Vol.8, e39643
- DOI
- 10.7554/eLife.39643
- PMID
- 31180325
- PMCID
- PMC6557630
- NLM abbreviation
- Elife
- ISSN
- 2050-084X
- eISSN
- 2050-084X
- Grant note
- DOI: 10.13039/100001391, name: Whitehall Foundation, award: 2014-08-03; DOI: 10.13039/100000001, name: National Science Foundation, award: 1557792; DOI: 10.13039/100000065, name: National Institute of Neurological Disorders and Stroke, award: R01NS085164; DOI: 10.13039/100000065, name: National Institute of Neurological Disorders and Stroke, award: T32NS007421 - PI Daniel T Tranel
- Language
- English
- Date published
- 06/10/2019
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute; Biology; Neuroscience and Pharmacology
- Record Identifier
- 9984070689802771
Metrics
16 Record Views