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Male‐specific alterations in structure of isolation call sequences of mouse pups with 16p11.2 deletion
Journal article   Open access   Peer reviewed

Male‐specific alterations in structure of isolation call sequences of mouse pups with 16p11.2 deletion

Swapna Agarwalla, Noelle S Arroyo, Natalie E Long, William T O'Brien, Ted Abel and Sharba Bandyopadhyay
Genes, brain and behavior, Vol.19(7), pp.e12681-n/a
09/2020
DOI: 10.1111/gbb.12681
PMCID: PMC7116069
PMID: 32558237
url
https://doi.org/10.1111/gbb.12681View
Published (Version of record) Open Access

Abstract

16p11.2 deletion is one of the most common gene copy variations that increases the susceptibility to autism and other neurodevelopmental disorders. This syndrome leads to developmental delays, including speech impairment and delays in expressive language and communication skills. To study developmental impairment of vocal communication associated with 16p11.2 deletion syndrome, we used the 16p11.2del mouse model and performed an analysis of pup isolation calls (PICs). The earliest PICs at postnatal day 5 from 16p11.2del pups were found altered in a male‐specific fashion relative to wild‐type (WT) pups. Analysis of sequences of ultrasonic vocalizations (USVs) emitted by pups using mutual information between syllables at different positions in the USV spectrograms showed that dependencies exist between syllables in WT mice of both sexes. The order of syllables was not random; syllables were emitted in an ordered fashion. The structure observed in the WT pups was identified and the pattern of syllable sequences was considered typical for the mouse line. However, typical patterns were totally absent in the 16p11.2del male pups, showing on average random syllable sequences, while the 16p11.2del female pups had dependencies similar to the WT pups. Thus, we found that PICs were reduced in number in male 16p11.2 pups and their vocalizations lack the syllable sequence order emitted by WT males and females and 16p11.2 females. Therefore, our study is the first to reveal sex‐specific perinatal communication impairment in a mouse model of 16p11.2 deletion and applies a novel, more granular method of analysing the structure of USVs. Structure in sequence of syllables in mouse pup isolation calls is present in wild‐type pups but is absent in a male specific manner in pups of the 16p11.2del mice, a model of autism spectrum disorders and communication impairment.
 vocalization sequence mutual information pup isolation calls USVs 16p11.2 deletion

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