Mammalian Homolog of Drosophila Tumor Suppressor Lethal (2) Giant Larvae Interacts with Basolateral Exocytic Machinery in Madin-Darby Canine Kidney Cells

Anne Muō̈sch; David Cohen; Charles Yeaman; W. James Nelson; Enrique Rodriguez-Boulan; Patrick J Brennwald

doi:10.1091/mbc.01-10-0496

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Mammalian Homolog of Drosophila Tumor Suppressor Lethal (2) Giant Larvae Interacts with Basolateral Exocytic Machinery in Madin-Darby Canine Kidney Cells

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Mammalian Homolog of Drosophila Tumor Suppressor Lethal (2) Giant Larvae Interacts with Basolateral Exocytic Machinery in Madin-Darby Canine Kidney Cells

Anne Muō̈sch, David Cohen, Charles Yeaman, W. James Nelson, Enrique Rodriguez-Boulan and Patrick J Brennwald

Molecular biology of the cell, Vol.13(1), pp.158-168

01/2002

DOI: 10.1091/mbc.01-10-0496

PMCID: PMC65098

PMID: 11809830

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Abstract

The Drosophila tumor suppressor protein lethal (2) giant larvae [l(2)gl] is involved in the establishment of epithelial cell polarity during development. Recently, a yeast homolog of the protein has been shown to interact with components of the post-Golgi exocytic machinery and to regulate a late step in protein secretion. Herein, we characterize a mammalian homolog of l(2)gl, called Mlgl, in the epithelial cell line Madin-Darby canine kidney (MDCK). Consistent with a role in cell polarity, Mlgl redistributes from a cytoplasmic localization to the lateral membrane after contact-naive MDCK cells make cell-cell contacts and establish a polarized phenotype. Phosphorylation within a highly conserved region of Mlgl is required to restrict the protein to the lateral domain, because a recombinant phospho-mutant is distributed in a nonpolar manner. Membrane-bound Mlgl from MDCK cell lysates was coimmunoprecipitated with syntaxin 4, a component of the exocytic machinery at the basolateral membrane, but not with other plasma membrane soluble N -ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins that are either absent from or not restricted to the basolateral membrane domain. These data suggest that Mlgl contributes to apico-basolateral polarity by regulating basolateral exocytosis.

Details

Title: Subtitle: Mammalian Homolog of Drosophila Tumor Suppressor Lethal (2) Giant Larvae Interacts with Basolateral Exocytic Machinery in Madin-Darby Canine Kidney Cells
Creators: Anne Muō̈sch - M. Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, New York 10021
David Cohen - M. Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, New York 10021
Charles Yeaman - M. Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, New York 10021
W. James Nelson - M. Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, New York 10021
Enrique Rodriguez-Boulan - M. Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, New York 10021
Patrick J Brennwald - M. Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, New York 10021
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.13(1), pp.158-168
DOI: 10.1091/mbc.01-10-0496
PMID: 11809830
PMCID: PMC65098
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology
Language: English
Date published: 01/2002
Academic Unit: Anatomy and Cell Biology; Internal Medicine
Record Identifier: 9984025309502771

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