Journal article
Mammalian germ-line transgenesis by transposition
Proceedings of the National Academy of Sciences - PNAS, Vol.99(7), pp.4495-4499
04/02/2002
DOI: 10.1073/pnas.062630599
PMCID: PMC123676
PMID: 11904379
Abstract
Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called
Sleeping Beauty
in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse.
Details
- Title: Subtitle
- Mammalian germ-line transgenesis by transposition
- Creators
- Adam J Dupuy - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentKarl Clark - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentCorey M Carlson - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentSabine Fritz - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentAnn E Davidson - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentKarra M Markley - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentKen Finley - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentColin F Fletcher - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentStephen C Ekker - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentPerry B Hackett - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentSandra Horn - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and DevelopmentDavid A Largaespada - The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and Development
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.99(7), pp.4495-4499
- DOI
- 10.1073/pnas.062630599
- PMID
- 11904379
- PMCID
- PMC123676
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 04/02/2002
- Academic Unit
- Anatomy and Cell Biology; Pathology
- Record Identifier
- 9984025411402771
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