Mapping ErbB receptors on breast cancer cell membranes during signal transduction

Shujie Yang; Mary Ann Raymond-Stintz; Wenxia Ying; Jun Zhang; Diane S Lidke; Stanly L Steinberg; Lance Williams; Janet M Oliver; Bridget S Wilson

doi:10.1242/jcs.007658

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Mapping ErbB receptors on breast cancer cell membranes during signal transduction

Journal article

Open access

Peer reviewed

Mapping ErbB receptors on breast cancer cell membranes during signal transduction

Shujie Yang, Mary Ann Raymond-Stintz, Wenxia Ying, Jun Zhang, Diane S Lidke, Stanly L Steinberg, Lance Williams, Janet M Oliver and Bridget S Wilson

Journal of cell science, Vol.120(Pt 16), pp.2763-2773

08/15/2007

DOI: 10.1242/jcs.007658

PMID: 17652160

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Abstract

Distributions of ErbB receptors on membranes of SKBR3 breast cancer cells were mapped by immunoelectron microscopy. The most abundant receptor, ErbB2, is phosphorylated, clustered and active. Kinase inhibitors ablate ErbB2 phosphorylation without dispersing clusters. Modest co-clustering of ErbB2 and EGFR, even after EGF treatment, suggests that both are predominantly involved in homointeractions. Heregulin leads to dramatic clusters of ErbB3 that contain some ErbB2 and EGFR and abundant PI 3-kinase. Other docking proteins, such as Shc and STAT5, respond differently to receptor activation. Levels of Shc at the membrane increase two- to five-fold with EGF, whereas pre-associated STAT5 becomes strongly phosphorylated. These data suggest that the distinct topography of receptors and their docking partners modulates signaling activities.

Transfection

Receptor, Epidermal Growth Factor - ultrastructure

Receptor, ErbB-3 - metabolism

Cricetulus

Humans

Receptor, ErbB-2 - metabolism

Phosphatidylinositol 3-Kinases - metabolism

Protein Transport - drug effects

Phosphotyrosine - metabolism

STAT5 Transcription Factor - metabolism

Breast Neoplasms - enzymology

Receptor, Epidermal Growth Factor - metabolism

Female

Cell Membrane - drug effects

CHO Cells

Shc Signaling Adaptor Proteins

Breast Neoplasms - ultrastructure

Cricetinae

Receptor, ErbB-2 - ultrastructure

Cell Membrane - ultrastructure

Neuregulin-1 - pharmacology

Receptor Protein-Tyrosine Kinases - metabolism

Cell Membrane - enzymology

Animals

Src Homology 2 Domain-Containing, Transforming Protein 1

Signal Transduction - drug effects

Receptor, ErbB-3 - ultrastructure

Epidermal Growth Factor - pharmacology

Adaptor Proteins, Signal Transducing - metabolism

Details

Title: Subtitle: Mapping ErbB receptors on breast cancer cell membranes during signal transduction
Creators: Shujie Yang - Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM 87131, USA
Mary Ann Raymond-Stintz
Wenxia Ying
Jun Zhang
Diane S Lidke
Stanly L Steinberg
Lance Williams
Janet M Oliver
Bridget S Wilson
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.120(Pt 16), pp.2763-2773
DOI: 10.1242/jcs.007658
PMID: 17652160
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Publisher: England
Grant note: P20 GM067594 / NIGMS NIH HHS R01 CA119232 / NCI NIH HHS
Language: English
Date published: 08/15/2007
Academic Unit: Pathology; Internal Medicine
Record Identifier: 9984046829902771

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