Mapping and quantification of the major oligosaccharide components of heparin

R J Linhardt; K G Rice; Y S Kim; D L Lohse; H M Wang; D Loganathan

doi:10.1042/bj2540781

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Mapping and quantification of the major oligosaccharide components of heparin

Journal article

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Mapping and quantification of the major oligosaccharide components of heparin

R J Linhardt, K G Rice, Y S Kim, D L Lohse, H M Wang and D Loganathan

The Biochemical journal, Vol.254(3), pp.781-787

09/15/1988

DOI: 10.1042/bj2540781

PMCID: PMC1135151

PMID: 3196292

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Abstract

A new method of determining the oligosaccharide composition of commercial glycosaminoglycan heparin is described in which heparin was first depolymerized using heparin lyase (EC 4.2.2.7), and then analysed by a single h.p.l.c. step. All 20 of the porcine and bovine heparins examined were found to contain a small number of major oligosaccharide components, which on average comprised 86% of their mass. The five most abundant oligosaccharides have defined chemical structures. Although the relative abundance of oligosaccharides varied, the heparins examined were surprisingly similar. Porcine, bovine, low-Mr, and high and low antithrombin III (ATIII)-affinity heparins, however, each had distinctly different proportions of these major oligosaccharide components. The concentrations of one of these five oligosaccharides, containing a portion of the ATIII binding site, correlated with the anticoagulant activity of the ATIII-affinity-fractionated porcine-mucosal heparins from which it was derived. An additional oligosaccharide of undetermined structure was found in significant quantities in both bovine heparin and high ATIII-affinity porcine-mucosal heparin. The correlation between oligosaccharide concentration and anticoagulant activity suggests that the oligosaccharide is derived from a structural variant of the ATIII-binding site. Finally, for the heparins examined chondroitin/dermatan sulphate formed 0.6-7.4% of their mass.

Details

Title: Subtitle: Mapping and quantification of the major oligosaccharide components of heparin
Creators: R J Linhardt - University of Iowa
K G Rice - University of Iowa
Y S Kim - University of Iowa
D L Lohse - University of Iowa
H M Wang - University of Iowa
D Loganathan - University of Iowa
Resource Type: Journal article
Publication Details: The Biochemical journal, Vol.254(3), pp.781-787
DOI: 10.1042/bj2540781
PMID: 3196292
PMCID: PMC1135151
NLM abbreviation: Biochem J
ISSN: 0264-6021
eISSN: 1470-8728
Language: English
Date published: 09/15/1988
Academic Unit: Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
Record Identifier: 9984366017002771

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