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Mapping self-associating chromatin hubs identifies Id proteins as key determinants of exhausted CD8 + T cell fate
Journal article   Peer reviewed

Mapping self-associating chromatin hubs identifies Id proteins as key determinants of exhausted CD8 + T cell fate

Wei Hu, Qing Chen, Shaoqi Zhu, Shengen Shawn Hu, Haibo Yu, Vanita Patel, Ying Wang, Vladimir P Badovinac, Yi Zhang, Chongzhi Zang, …
Nature immunology
07/06/2026
DOI: 10.1038/s41590-026-02578-4
PMID: 42409948

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Abstract

Within days of exposure to chronic viral infections, activated CD8 T cells differentiate into Tcf1 Slamf6 Tim3 exhaustion-prone effector T (T ) cells or self-renewing Tcf1 Slamf6 Tim3 precursor exhausted T (T ) cells. Here we showed that early CD8 T cell fates were imprinted by forming subset-specific, self-associating chromatin hubs. Chromatin hub assembly coincided with effector or stemness gene induction and identified the transcription cofactors Id2 and Id3 as key regulators that promoted CD8 T and CD8 T cell fates, respectively. Id2 drove CD8 T cell specification by activating effector genes, while suppressing genes involved in exhaustion and stemness. In contrast, Id3-repressed effector genes but upregulated IL-7Rα and AhR, thereby maintaining the CD8 T cell pool. Mechanistically, Id2 and Id3 exhibited a distinct impact on the chromatin accessibility landscape in early CD8 T cells by engaging Runx3 and Tcf1 transcription factors along with E proteins. These findings indicated that reshaping chromatin architecture represents a critical means for specifying CD8 T cell fates and ensuring lineage stability.

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