Mapping the redox state of CHOP-treated non-Hodgkin's lymphoma xenografts in mice

He N Xu; Tahreem A Mir; Seung-Cheol Lee; Min Feng; Namisa Farhad; Regine Choe; Jerry D Glickson; Lin Z Li

doi:10.1007/978-1-4614-7411-1_33

Back

Mapping the redox state of CHOP-treated non-Hodgkin's lymphoma xenografts in mice

Journal article

Peer reviewed

Mapping the redox state of CHOP-treated non-Hodgkin's lymphoma xenografts in mice

He N Xu, Tahreem A Mir, Seung-Cheol Lee, Min Feng, Namisa Farhad, Regine Choe, Jerry D Glickson and Lin Z Li

Advances in experimental medicine and biology, Vol.789, pp.243-249

2013

DOI: 10.1007/978-1-4614-7411-1_33

PMCID: PMC4526262

PMID: 23852501

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/4526262View

Open Access

Abstract

Drug treatment may alter the metabolism of cancer cells and may alter the mitochondrial redox state. Using the redox scanner that collects the fluorescence signals from both the oxidized flavoproteins (Fp) and the reduced form of nicotinamide adenine dinucleotide (NADH) in snap-frozen tumor tissues, we investigated the effects of chemotherapy on mouse xenografts of a human diffuse large B-cell lymphoma cell line (DLCL2). The mice in the treatment group were treated with CHOP - cyclophosphamide (C) + hydroxydoxorubicin (H) + Oncovin (O) + prednisone (P) using the following regimen: CHO administration on day 1 followed by prednisone administration on day 1-5. On day 5 the mitochondrial redox state of the treated group was slightly more reduced than that of the control group (p = 0.049), and the Fp content of the treated group was significantly decreased (p = 0.033).

Animals

Antineoplastic Combined Chemotherapy Protocols - administration & dosage

Antineoplastic Combined Chemotherapy Protocols - pharmacology

Cell Line, Tumor

Cyclophosphamide - administration & dosage

Doxorubicin - administration & dosage

Female

Humans

Lymphoma, Large B-Cell, Diffuse - drug therapy

Lymphoma, Large B-Cell, Diffuse - metabolism

Lymphoma, Non-Hodgkin - drug therapy

Lymphoma, Non-Hodgkin - metabolism

Mice

Mice, Nude

Mitochondria - drug effects

Mitochondria - metabolism

NAD - metabolism

Oxidation-Reduction

Prednisone - administration & dosage

Vincristine - administration & dosage

Xenograft Model Antitumor Assays

Details

Title: Subtitle: Mapping the redox state of CHOP-treated non-Hodgkin's lymphoma xenografts in mice
Creators: He N Xu - University of Pennsylvania
Tahreem A Mir - University of Pennsylvania
Seung-Cheol Lee - University of Pennsylvania
Min Feng - University of Pennsylvania
Namisa Farhad - Johnson Foundation
Regine Choe - University of Rochester
Jerry D Glickson - University of Pennsylvania
Lin Z Li - University of Pennsylvania
Resource Type: Journal article
Publication Details: Advances in experimental medicine and biology, Vol.789, pp.243-249
DOI: 10.1007/978-1-4614-7411-1_33
PMID: 23852501
PMCID: PMC4526262
NLM abbreviation: Adv Exp Med Biol
eISBN: 1461474116; 9781461474111
ISSN: 0065-2598
eISSN: 2214-8019
Grant note: P41 RR002305 / NCRR NIH HHS R01 CA101700 / NCI NIH HHS K99 CA126187 / NCI NIH HHS P41RR02305 / NCRR NIH HHS 2U24-CA083105 / NCI NIH HHS K99/R00-CA126187 / NCI NIH HHS U24 CA083105 / NCI NIH HHS 2R01-CA101700 / NCI NIH HHS
Language: English
Date published: 2013
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9984756265502771

Metrics

4 Record Views

5 Times Cited - Web of Science