Journal article
Mapping the spatial transcriptomic signature of the hippocampus during memory consolidation
Nature communications, Vol.14(1), 6100
09/29/2023
DOI: 10.1038/s41467-023-41715-7
PMCID: PMC10541893
PMID: 37773230
Abstract
Memory consolidation involves discrete patterns of transcriptional events in the hippocampus. Despite the emergence of single-cell transcriptomic profiling techniques, mapping the transcriptomic signature across subregions of the hippocampus has remained challenging. Here, we utilized unbiased spatial sequencing to delineate transcriptome-wide gene expression changes across subregions of the dorsal hippocampus of male mice following learning. We find that each subregion of the hippocampus exhibits distinct yet overlapping transcriptomic signatures. The CA1 region exhibited increased expression of genes related to transcriptional regulation, while the DG showed upregulation of genes associated with protein folding. Importantly, our approach enabled us to define the transcriptomic signature of learning within two less-defined hippocampal subregions, CA1 stratum radiatum, and oriens. We demonstrated that CA1 subregion-specific expression of a transcription factor subfamily has a critical functional role in the consolidation of long-term memory. This work demonstrates the power of spatial molecular approaches to reveal simultaneous transcriptional events across the hippocampus during memory consolidation.
Transcriptional events are critical for hippocampus-dependent long-term memory storage. Here, the authors utilize spatial transcriptomics to elucidate localized gene expression patterns across hippocampal subregions during memory consolidation.
Details
- Title: Subtitle
- Mapping the spatial transcriptomic signature of the hippocampus during memory consolidation
- Creators
- Yann Vanrobaeys - University of IowaUtsav Mukherjee - University of IowaLucy Langmack - University of IowaStacy E. Beyer - University of IowaEthan Bahl - University of IowaLi-Chun Lin - University of IowaJacob J. Michaelson - University of IowaTed Abel - University of IowaSnehajyoti Chatterjee - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Nature communications, Vol.14(1), 6100
- DOI
- 10.1038/s41467-023-41715-7
- PMID
- 37773230
- PMCID
- PMC10541893
- NLM abbreviation
- Nat Commun
- ISSN
- 2041-1723
- eISSN
- 2041-1723
- Publisher
- Nature Publishing Group UK
- Grant note
- ; R01 MH 087463 / ; (HAWK-IDDRC) P50 HD103556 / ; K99/R00 AG068306 / ;
- Language
- English
- Date published
- 09/29/2023
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Neurology; Communication Sciences and Disorders; Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984473618302771
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